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Gene expression in wild-type and transgenic plants overexpressing rice OsTOP6A1 gene

ABSTRACT: 10-day-old wild-type and homozygous transgenic Arabidopsis seedlings (overexpressing rice topoisomerase 6 subunit A1; OsTOP6A1) grown under normal growth conditions were used for total RNA isolation. The 5 micrograms of each total RNA sample was processed for microarray analysis according to Affymetrix protocol. Experiment Overall Design: The experiment involves the study of differential gene expression in transgenic Arabidopsis seedlings overexpressing rice topoisomerase 6 subunit A1 (OsTOP6A1) cDNA as compared to wild-type.Three biological replicates for each sample were used for microarray analysis.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Mukesh Jain

PROVIDER: E-GEOD-6812 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Constitutive expression of a meiotic recombination protein gene homolog, OsTOP6A1, from rice confers abiotic stress tolerance in transgenic Arabidopsis plants.

Jain Mukesh M Tyagi Akhilesh K AK Khurana Jitendra P JP

Plant cell reports 20071211 4

Plant productivity is greatly influenced by various environmental stresses, such as high salinity and drought. Earlier, we reported the isolation of topoisomerase 6 homologs from rice and showed that over expression of OsTOP6A3 and OsTOP6B confers abiotic stress tolerance in transgenic Arabidopsis plants. In this study, we have assessed the function of nuclear-localized topoisomerase 6 subunit A homolog, OsTOP6A1, in transgenic Arabidopsis plants. The over expression of OsTOP6A1 in transgenic Ar ...[more]

PMID: 18071708

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