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Gene expression changes in a histone sprocket arginine mutant in histone H2A R78A


ABSTRACT: Examined gene expression changes in a histone H2A R78A mutant in Saccharomyces cerevisiae relative to wild-type cells. THe overall goal of this study was to determine the functions of histone 'sprocket' arginine residues, which insert into the DNA minor groove in the nucleosome. We examined the roles of sprocket arginine mutants in gene expression, histone incorporation, and DNA repair. Three wild-type control replicate samples and three experimental (H2A R78A) replicates

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: John Wyrick 

PROVIDER: E-GEOD-68159 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Histone Sprocket Arginine Residues Are Important for Gene Expression, DNA Repair, and Cell Viability in Saccharomyces cerevisiae.

Hodges Amelia J AJ   Gallegos Isaura J IJ   Laughery Marian F MF   Meas Rithy R   Tran Linh L   Wyrick John J JJ  

Genetics 20150512 3


A critical feature of the intermolecular contacts that bind DNA to the histone octamer is the series of histone arginine residues that insert into the DNA minor groove at each superhelical location where the minor groove faces the histone octamer. One of these "sprocket" arginine residues, histone H4 R45, significantly affects chromatin structure in vivo and is lethal when mutated to alanine or cysteine in Saccharomyces cerevisiae (budding yeast). However, the roles of the remaining sprocket arg  ...[more]

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