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RNAseq and Lariat sequencing of different S. pombe growth conditions

ABSTRACT: We performed lariat sequencing to profile the diversity of spliced RNA lariats in S. pombe identify annotated and alternate introns. Three different growth conditions were used to grow S. pombe wt and S. pombe Δdbr1. Lariat sequencing of the Δdbr1 strains and RNAseq of the wt and Δdbr1 strains were done to profile intron lariats and exon-exon junctions in RNA transcripts.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Jeffrey Pleiss

PROVIDER: E-GEOD-68345 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Widespread alternative and aberrant splicing revealed by lariat sequencing.

Stepankiw Nicholas N Raghavan Madhura M Fogarty Elizabeth A EA Grimson Andrew A Pleiss Jeffrey A JA

Nucleic acids research 20150810 17

Alternative splicing is an important and ancient feature of eukaryotic gene structure, the existence of which has likely facilitated eukaryotic proteome expansions. Here, we have used intron lariat sequencing to generate a comprehensive profile of splicing events in Schizosaccharomyces pombe, amongst the simplest organisms that possess mammalian-like splice site degeneracy. We reveal an unprecedented level of alternative splicing, including alternative splice site selection for over half of all ...[more]

PMID: 26261211

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