Unknown,Transcriptomics,Genomics,Proteomics

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ChIPseq analyses in germinal center B cells


ABSTRACT: Germinal center B cells were isolated from human tonsil tissue and crosslinked. ChIP was performed on two distinct pools of germinal center cells, each obtained from 3-5 donors. The experiment includes two biological replicates (germinal center cell pools from different donors). ChIP was performed on both pools and subject to library preparation and sequencing. Input DNA was sequenced for both pools.

ORGANISM(S): Homo sapiens

SUBMITTER: Riccardo Dalla-Favera 

PROVIDER: E-GEOD-68349 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The FOXO1 Transcription Factor Instructs the Germinal Center Dark Zone Program.

Dominguez-Sola David D   Kung Jennifer J   Holmes Antony B AB   Wells Victoria A VA   Mo Tongwei T   Basso Katia K   Dalla-Favera Riccardo R  

Immunity 20151124 6


The pathways regulating formation of the germinal center (GC) dark zone (DZ) and light zone (LZ) are unknown. In this study we show that FOXO1 transcription factor expression was restricted to the GC DZ and was required for DZ formation, since its absence in mice led to the loss of DZ gene programs and the formation of LZ-only GCs. FOXO1-negative GC B cells displayed normal somatic hypermutation but defective affinity maturation and class switch recombination. The function of FOXO1 in sustaining  ...[more]

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