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MiRNA expression profile in human CD34+/CD105- cells derived from the renal artery subsequent to long warm ischemia compared short warm ischemia

ABSTRACT: MiRNA regulate the maintenance, differentiation and function of stem cells and progenitor cells. miRNA expression of progenitor cells located in the adventital layer of arterial vessels has not been characterized in either animal or human models. Further it is unknown if local arterial miRNA expression profiles change after injury of end organs supplied blood by these arterial conduits. CD34+/CD105- cells were extracted and analyzed for changes in miRNA expression after kidney specific ischemic injury. CD34+/CD105- cells were isolated from renal artery after short warm ischemic time in living donor kidney explants and long warm ischemic time following radical nephrectomy for renal cell cancer

ORGANISM(S): Homo sapiens

SUBMITTER: Andrew Siedlecki

PROVIDER: E-GEOD-68366 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MiRNA expression profile in mouse CD34+/CD105- cells derived from the renal artery subsequent to renal ischemia/reperfusion injury

Project description:MiRNA regulate the maintenance, differentiation and function of stem cells and progenitor cells. miRNA expression of progenitor cells located in the adventital layer of arterial vessels has not been characterized in either animal or human models. Further it is unknown if local arterial miRNA expression profiles change after injury of end organs supplied blood by these arterial conduits. CD34+/CD105- cells were extracted and analyzed for changes in miRNA expression after kidney specific ischemic injury. CD34+/CD105- cells were isolated from mouse renal artery after microvascular clamping of renal arteries bilaterally

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MiRNA expression profile in human CD34+/CD105- cells derived from the renal artery subsequent to long warm ischemia compared short warm ischemia

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MiRNA expression profile in mouse CD34+/CD105- cells derived from the renal artery subsequent to renal ischemia/reperfusion injury

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The analysis of differential gene expression after remote ischemic preconditioning of renal ischemia reperfusion injury

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Project description:MiRNA expression profile in CD34+/CD105- cells derived from the renal artery after kidney specific ischemic injury.

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Homo sapiens

Project description:MiRNA expression profile in human CD34+/CD105- cells derived from the renal artery subsequent to long warm ischemia compared short warm ischemia

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Cardiac-dead mouse lungs may remain transplantable at 37ºC for 4 hours if gas exchange is preserved

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