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Differential expression of genes in Streptococcus sanguinis nox mutant and its complemented strain

ABSTRACT: Transcriptional profiling of Streptococcus sanguinis nox mutant and its complemented strain compared to the wild-type SK36. Two experiments:nox mutant vs. SK36, nox complemeted strain vs. SK36. Biological replicates: triplicates, independently grown and harvested. Four replicate per array.

ORGANISM(S): Streptococcus sanguinis

SUBMITTER: Xiuchun Ge

PROVIDER: E-GEOD-68492 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Streptococcus sanguinis gene expression at late-log growth phase

Project description:We found that many of the genes for which mutants could not be obtained using promoterless aphA-3 gene replacement were annotated as acquired via horizontal gene transfer or as encoding hypothetical proteins. We were curious about the explanation of this finding. The expression of these genes was therefore examined by microarray analysis. The microarray data showed many had undetectable expression. This led us to suspect that many of the unrecoverable mutants might have resulted from insufficient expression of the promoterless aphA-3 gene. S. sanguinis cultures at late log growth phase at 37 oC under microaerobic condition were used for microarray analysis. RNA from each of three independent samples was isolated by RNeasy mini kit (Qiagen, Valencia, CA). Spotted microarray slides were obtained from the Pathogen Functional Genomics Resource Center at JCVI. Each gene had four repeats in one spotted microarray slides. The microarray was performed according to the manufacturerM-bM-^@M-^Ys protocol. Each sample was divided into two parts and labeled by Cy5 and Cy3 dyes respectively for microarray. The microarray data were analyzed by programs TIGR Spotfinder and Midas to obtain the ratio of one dye to another dye for gene relative expression. Additionally, the ratio of dye intensity to background in a microarray slide was obtained after Spotfinder analysis. Absolute expression of each gene was represented by the average ratio of dye intensity to background for each slide.

2013-11-01 | E-GEOD-25340 | biostudies-arrayexpress

Differential expression of genes in Streptococcus sanguinis nox mutant and its complemented strain

Project description:Transcriptional profiling of Streptococcus sanguinis nox mutant and its complemented strain compared to the wild-type SK36.

2015-05-04 | GSE68492 | GEO

spxA1-null mutant of Streptococcus sanguinis SK36

Project description:Transcriptional profiling of the spxA1-null mutant of Streptococcus sanguinis SK36 compared with wild type. The spxA1 gene was inactivated in Streptococcus sanguinis SK36, and the mutant demonstrated opaque colony morphology, reduced hydrogen peroxide (H2O2) production, and reduced antagonistic activity against Streptococcus S. mutans UA159 both on plates and in liquid media. The mutant also showed decreased tolerance to high temperature, and acidic and oxidative stresses. Complementation of the ΔspxA1 mutant with spxA1 restored colony morphology, H2O2 production and stress tolerance to the ΔspxA1 mutant. The mutant also exhibited an ~5-fold reduction in competitiveness in an animal model of endocarditis, indicating the involvement of SpxA1 in endocarditis virulence. Microarray studies revealed that a number of SpxA1-upregulated genes are involved in oxidative stress. The expression of spxB and nox (which encode pyruvate oxidase and NADH oxidase, respectively, and are involved in H2O2 production and nox involved virulence) significantly decreased in ΔspxA1 compared with the wild type. This may be at least partly responsible for the decreased H2O2 production and reduced virulence in the ΔspxA1 mutant because spxB and nox were involved in H2O2 production and nox involved virulence. One-condition experiment: ΔspxA1 vs. S. sanguinis SK36 cells. Biological replicates: 3 wild type, 3 ΔspxA1, independently grown and harvested. One replicate (one wild type and one ΔspxA1 mixture) per array.

2011-12-08 | E-GEOD-34203 | biostudies-arrayexpress

ssa_1972-null mutant of Streptococcus sanguinis SK36

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2013-07-17 | E-GEOD-48940 | biostudies-arrayexpress

spxA1-null mutant of Streptococcus sanguinis SK36

2011-12-08 | GSE34203 | GEO

ssa_1972-null mutant of Streptococcus sanguinis SK36

2013-07-17 | GSE48940 | GEO

Analysis of differential gene expression in the Streptococcus sanguinis SSA_0351 mutant

Project description:Gene expression profile of the Streptococcus sanguinis SSA_0351 deletion mutant, ssx_0351, compared to the wild-type strain SK36

2019-06-01 | GSE90021 | GEO

Analysis of differential gene expression in the Streptococcus sanguinis brpL mutant

Project description:Gene expression profile of the Streptococcus sanguinis transcription regulator, brpL, deletion mutant compared to the wild-type strain SK36

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Analysis of differential gene expression in the Streptococcus sanguinis SK36 treated with heat shock for 3 periods of time

Project description:Gene expression profile of the Streptococcus sanguinis SK36 exposed to heat (43 C) for three time periods compared to the untreated strain SK36.

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Analysis of differential gene expression in the Streptococcus sanguinis ciaR mutant

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