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Transportome profiling identifies profound alterations in Crohn’s disease partially restored by commensal bacteria

ABSTRACT: In the present study, the transcriptional analysis of CD biopsies reveals profound alterations in the ileum transportome profile. More than 60 SLC transporters showed different expression pattern compared with the healthy donors, being mostly decreased. Changes were confirmed in almost all the eighteen altered SLCs analyzed by RT-PCR. The results obtained display alterations in amino acid transporters, purinome members, Zn transporters and metallothioneins. All together, these alterations which mainly involve transporters localized at the apical membrane of the enterocyte anticipate impaired amino acid uptake and purinergic responses. Remarkably, incubation of explants with specific commensal bacteria restored almost all CD transportome alterations. Genome wide transcriptomic profile of Ileal mucosa from five healthy and six Chron's disease samples .

ORGANISM(S): Homo sapiens

SUBMITTER: Juanjo Lozano 

PROVIDER: E-GEOD-68570 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transportome Profiling Identifies Profound Alterations in Crohn's Disease Partially Restored by Commensal Bacteria.

Pérez-Torras Sandra S   Iglesias Ingrid I   Llopis Marta M   Lozano Juan J JJ   Antolín María M   Guarner Francisco F   Pastor-Anglada Marçal M  

Journal of Crohn's & colitis 20160213 7

<h4>Background and aims</h4>Several transport alterations have been described in intestinal inflammatory diseases. This is relevant because the primary function of the intestine is nutrient and mineral absorption. However, analysis of the transportome as a whole and the effect of commensal bacteria on it have not been addressed so far.<h4>Methods</h4>Five healthy and 6 Crohn's disease (CD) samples were hybridized to human HT-12 V4 Illumina GeneChip. Results were validated by reverse transcriptio  ...[more]

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