Genome-wide maps of nuclear lamina interactions in single human cells (CEL-seq)

ABSTRACT: Mammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large Lamina Associated Domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of ~400 maps reveals a core architecture of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts are more sensitive to a change in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination of NL contacts, even over tens of megabases. Such coordinated loci exhibit preferential interactions as detected by Hi-C. Finally, single-cell gene e

ORGANISM(S): Homo sapiens

SUBMITTER: Siddharth Dey 

PROVIDER: E-GEOD-68596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress