Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human peripheral blood lymphocytes: comparison between melanoma patients and healthy controls


ABSTRACT: We focused on the major peripheral blood lymphocyte populations that may be involved in anti-tumor responses and negatively impacted by cancer, specifically CD8 T cells, CD4 T cells, B cells and CD56dim natural killer cells. The pure cell subsets were stringently sorted by flow cytometry from PBMC samples. Gene expression profiles of these cell populations from melanoma patients were compared to healthy controls. Experiment Overall Design: The raw data set contained 48 arrays: 6 healthy and 6 melanoma arrays for each of the 4 cell types. Two of the arrays had quality issues due to background noise and were excluded, leaving us with 46 arrays. Experiment Overall Design: On each array we used Cy3 to label a pool of two RNA samples from a pair of age and gender matched stage IV (American Joint Committee on Cancer) melanoma patients or from a pair of age and gender matched healthy donors. We used Cy5 to label the Total Lymphocyte Reference (TLR) RNA. The TLR is a common reference specifically created for this study using the total peripheral lymphocyte fraction from 20 healthy donors.

ORGANISM(S): Homo sapiens

SUBMITTER: Peter Lee 

PROVIDER: E-GEOD-6887 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Down-regulation of the interferon signaling pathway in T lymphocytes from patients with metastatic melanoma.

Critchley-Thorne Rebecca J RJ   Yan Ning N   Nacu Serban S   Weber Jeffrey J   Holmes Susan P SP   Lee Peter P PP  

PLoS medicine 20070501 5


<h4>Background</h4>Dysfunction of the immune system has been documented in many types of cancers. The precise nature and molecular basis of immune dysfunction in the cancer state are not well defined.<h4>Methods and findings</h4>To gain insights into the molecular mechanisms of immune dysfunction in cancer, gene expression profiles of pure sorted peripheral blood lymphocytes from 12 patients with melanoma were compared to 12 healthy controls. Of 25 significantly altered genes in T cells and B ce  ...[more]

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