Unknown,Transcriptomics,Genomics,Proteomics

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Tumor exosome integrins determine organotropic metastasis


ABSTRACT: Stephen Paget first proposed, in 1889, that organ distribution of metastases is a non-random event, yet metastatic organotropism remains one of the greatest mysteries in cancer biology. Here, we demonstrate that exosomes released by lung-, liver- and brain-tropic tumor cells fuse preferentially with resident cells at their predicted destination, such as fibroblasts and epithelial cells in the lung, Kupffer cells in the liver, and endothelial cells in the brain. We found that exosome homing to organ-specific cell types prepares the pre-metastatic niche and that treatment with exosomes derived from lung tropic models can redirect metastasis to the lung. Proteomic profiling of exosomes revealed distinct integrin expression patterns associated with each organ-specific metastasis. Whereas exosomal integrins α6β4 and α6β1 were associated with lung metastasis, exosomal integrins αvβ5 and αvβ3 were linked with liver and brain metastases, respectively. Targeting α6β4 and αvβ5 integrins decreased exosome uptake and metastasis in the lung and liver, respectively. Importantly, we demonstrate that exosome uptake activates a cell-specific subset of S100 family genes, known to support cell migration and niche formation. Finally, our clinical data indicate that integrin-expression profiles in circulating plasma exosomes from cancer patients could be used to predict organ-specific metastasis. Education of human von Kupffer cells in vitro with human pancreatic cancer exosomes

ORGANISM(S): Homo sapiens

SUBMITTER: David Lyden 

PROVIDER: E-GEOD-68919 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver.

Costa-Silva Bruno B   Aiello Nicole M NM   Ocean Allyson J AJ   Singh Swarnima S   Zhang Haiying H   Thakur Basant Kumar BK   Becker Annette A   Hoshino Ayuko A   Mark Milica Tešić MT   Molina Henrik H   Xiang Jenny J   Zhang Tuo T   Theilen Till-Martin TM   García-Santos Guillermo G   Williams Caitlin C   Ararso Yonathan Y   Huang Yujie Y   Rodrigues Gonçalo G   Shen Tang-Long TL   Labori Knut Jørgen KJ   Lothe Inger Marie Bowitz IM   Kure Elin H EH   Hernandez Jonathan J   Doussot Alexandre A   Ebbesen Saya H SH   Grandgenett Paul M PM   Hollingsworth Michael A MA   Jain Maneesh M   Mallya Kavita K   Batra Surinder K SK   Jarnagin William R WR   Schwartz Robert E RE   Matei Irina I   Peinado Héctor H   Stanger Ben Z BZ   Bromberg Jacqueline J   Lyden David D  

Nature cell biology 20150518 6


Pancreatic ductal adenocarcinomas (PDACs) are highly metastatic with poor prognosis, mainly due to delayed detection. We hypothesized that intercellular communication is critical for metastatic progression. Here, we show that PDAC-derived exosomes induce liver pre-metastatic niche formation in naive mice and consequently increase liver metastatic burden. Uptake of PDAC-derived exosomes by Kupffer cells caused transforming growth factor β secretion and upregulation of fibronectin production by he  ...[more]

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