Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human Calu-3 cell micro RNA transcriptome response to a wild type infectious clone of H7N9 Influenza virus and mutant H7N9 viruses

ABSTRACT: The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in human Calu-3 cells infected with WT A/Anhui/1/2013 (H7N9; 'AH1'), and AH - NS1-103F/106M. Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 2 days before infection. Prior to infection, washed cells 2 times with PBS, and then infected with a multiplicity of infection of 1. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 7 and 12 h post-infection

ORGANISM(S): Homo sapiens

SUBMITTER: Yoshihiro Kawaoka

PROVIDER: E-GEOD-69027 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Human Calu-3 cell transcriptome response to a wild type infectious clone of H7N9 Influenza virus and mutant H7N9 viruses

Project description:The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in human Calu-3 cells infected with WT A/Anhui/1/2013 (H7N9; 'AH1'), AH - NS1-103F/106M, and AH1 - 691 (ferret adapted virus). Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 2 days before infection. Prior to infection, washed cells 2 times with PBS, and then infected with a multiplicity of infection of 1. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 7, 12 and 24 h post-infection

2015-05-20 | E-GEOD-69026 | biostudies-arrayexpress

Human Calu-3 cell transcriptome response to a wild type infectious clone of H5N1 Influenza virus and mutant H5N1 viruses [miRNA]

Project description:The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in human Calu-3 cells infected with wild-type A/Vietnam/1203/2004 (H5N1; 'VN1203'; derived by reverse genetics), PB2-K627E (627K is a mammalian-adapting mutation that increases polymerase replicatve activity; '627E' is typically found in avian species), and NS1-trunc124 (this 'NS1' protein has a 90 amino acid truncation at the C-terminus). Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 2 days before infection. Prior to infection, washed cells 2 times with PBS, and then infected with a multiplicity of infection of 1. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 7, 12, and 24 hr post-infection.

2016-02-20 | E-GEOD-76600 | biostudies-arrayexpress

Primary human microvascular endothelial cell transcriptome (microRNA) response to wild type Mers-CoV (icMERS-CoV EMC2012)

Project description:The purpose is to obtain samples for microRNA analysis in primary human microvascular endothelial cells infected with wild type Mers-CoV (icMERS). Human microvascular endothelial cells were infected with a multiplicity of infection of 5 PFU per cell. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 12 and 24 h post-infection. MERSCOV002.0P references the write up of the experimental procedure.

2016-04-06 | E-GEOD-79216 | biostudies-arrayexpress

Primary human microvascular endothelial cell transcriptome (mRNA) response to a wild type Mers-CoV (icMERS-CoV EMC2012)

Project description:The purpose is to obtain samples for mRNA analysis in primary human microvascular endothelial cells infected with wild type Mers-CoV (icMERS). Human microvascular endothelial cells were infected with a multiplicity of infection of 5 PFU per cell. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 12, 24, 36, and 48 h post-infection. MERSCOV002.0P references the write up of the experimental procedure.

2016-03-25 | E-GEOD-79218 | biostudies-arrayexpress

Human Calu-3 cell transcriptome response to human interferon α [mRNA]

Project description:The purpose is to obtain samples for mRNA and miRNA analysis with and without interferon α treatment; in parallel, to obtain cell pellets to forward to PNNL for use in top-down proteomics pilot experiments Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 3 days before infection. Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 500 U/ml of human interferon α (PBL Biosciences). Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points. Infected samples were collected in quadruplet; time-matched mocks were collected in quadruplet in parallel with infected samples. Time points: 6, 12, and 18 h post-infection

2015-06-25 | E-GEOD-70217 | biostudies-arrayexpress

Human Calu-3 cell transcriptome response to human interferon α [miRNA]

2015-06-25 | E-GEOD-70220 | biostudies-arrayexpress

Human U937 cell transcriptome response to Zaire Ebola virus wild-type in the delta-VP30 background and delta-mucin virus

Project description:The purpose is to obtain samples for mRNA analysis in human U937 cells infected with Zaire Ebola virus wild-type in the deltaVP30 background and delta-mucin virus. Human U937 cells (monocyte-like) expressing the Ebola VP30 protein were infected with Zaire Ebola virus wild-type (wild) in the delta-VP30 background and delta-mucin virus (encodes a GP lacking the mucin domain) (mucin). Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 8, 18, and 30 h post-infection.

2016-05-19 | E-GEOD-80832 | biostudies-arrayexpress

Mouse cerebellum transcriptome response to a wild type West Nile virus (WNV), New York 99 Strain, and mutant WNV-E218A viruses [WCB001_microRNA]

Project description:The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse cerebellum infected with wild-type West Nile virus (WNV; WNV-NY99 382), and mutant WNV-E218A (WNV-NY99 382 E218A 2 nt). Mice were inoculated with WNV intracranially at a dose of 100 FFU. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 2 and 4 days post-infection. GSE77161 (WCT001) and this study (WCB001) are 1/4 of cortex and 1/2 of cerebellum from the same mice. Tissues from this experiment are matched to tissues from GSE77161 (WCT001). Infectivity (titer) and pathology are measured from cortex tissue in GSE77161 (WCT001).

2016-02-21 | E-GEOD-77160 | biostudies-arrayexpress

Mouse cortex transcriptome response to a wild type West Nile virus (WNV), New York 99 Strain, and mutant WNV-E218A viruses [WCT001_miRNA]

Project description:The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse cortex infected with wild-type West Nile virus (WNV; WNV-NY99 382), and mutant WNV-E218A (WNV-NY99 382 E218A 2 nt). Mice were inoculated with WNV intracranially at a dose of 100 FFU. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 2 and 4 days post-infection. This study (WCT001) and GSE77160 (WCB001) are 1/4 of cortex and 1/2 of cerebellum from the same mice. Tissues from this experiment are matched to tissues from GSE77160 (WCB001). Infectivity (titer) and pathology are measured from the tissue in WCT001 (i.e., this experiment).

2016-03-12 | E-GEOD-77161 | biostudies-arrayexpress

Mouse cerebellum transcriptome response to a wild type West Nile virus (WNV), New York 99 Strain, and mutant WNV-E218A viruses [WCB001_mRNA]

Project description:The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse cerebellum infected with wild-type West Nile virus (WNV; WNV-NY99 382), and mutant WNV-E218A (WNV-NY99 382 E218A 2 nt). Mice were inoculated with WNV intracranially at a dose of 100 FFU. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 1, 2, 4, and 6 days post-infection. GSE77193 (WCT001) and this study (WCB001) are 1/4 of cortex and 1/2 of cerebellum from the same mice. Tissues from this experiment are matched to tissues from GSE77193 (WCT001). Infectivity (titer) and pathology are measured from cortex tissue in GSE77193 (WCT001).

2016-02-15 | E-GEOD-77192 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data