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Transcriptome profiling of differentially expressed genes from floral buds and flowers of male sterile and fertile lines in watermelon


ABSTRACT: Male sterility is important mechanism in watermelon for production of hybrid seed. While some fruit development related studies were widely performed in watermelon, there are no reports of profiling gene expression in floral organs of watermelon. RNA-seq analysis was performed in order to identify male sterility related genes from two different groups of watermelon (genetic male-sterile (GMS) DAH3615-MS line and male-fertile DAH3615 line, respectively) to identify the differentially expressed genes (DEGs). This study employed tophat and edgeR for transcriptome analysis of next-generation RNA-seq data, which included 2 tissues obtained from 2 different breeds of watermelon

ORGANISM(S): Citrullus lanatus

SUBMITTER: Minseok Seo 

PROVIDER: E-GEOD-69073 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome profiling of differentially expressed genes in floral buds and flowers of male sterile and fertile lines in watermelon.

Rhee Sun-Ju SJ   Seo Minseok M   Jang Yoon-Jeong YJ   Cho Seoae S   Lee Gung Pyo GP  

BMC genomics 20151109


<h4>Background</h4>Male sterility is an important mechanism for the production of hybrid seeds in watermelon. Although fruit development has been studied extensively in watermelon, there are no reports on gene expression in floral organs. In this study, RNA-sequencing (RNA-seq) was performed in two near-isogenic watermelon lines (genic male sterile [GMS] line, DAH3615-MS and male fertile line, DAH3615) to identify the differentially expressed genes (DEGs) related to male sterility.<h4>Results</h  ...[more]

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