Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Expression profiles of murine FOXO1+/+ and FOXO1-null GC B cells


ABSTRACT: The pathways regulating the formation of the germinal center (GC) dark- (DZ) and light- (LZ) zones are unknown. We show that FOXO1 expression is restricted to the GC DZ and is required for DZ formation, since its absence in mice leads to the complete loss of DZ gene programs and the formation of LZ-only GCs. FOXO1-negative GC B-cells display normal somatic hypermutation, but defective affinity maturation and class switch recombination. The function of FOXO1 in sustaining the DZ program involves the transactivation of the chemokine receptor CXCR4, and the cooperation with BCL6 in the trans-repression of genes involved in immune activation, DNA-repair and plasma cell differentiation. These results have also implications for understanding the role of FOXO1 mutations in lymphomagenesis. We used microarrays to determine the consequences of FOXO1 deletion in the GC B cell comparment, and correlate these data with phenotypic changes GC B cell subpopulations were collected by Fluorescence Activated Cell Sorting (FACS) from B cell enriched fractions of splenic mononuclear cell pools (12 days after SRBC immunization). 20ng of total RNA (RIN>9) for each sample was used as a template for linear cDNA amplification (Ovation RNA amplification Kit, NuGen). cDNA was labeled using the Encore Biotin Labeling Kit (NuGen) and hybridized to Affymetrix Mouse 430.2 gene expression arrays

ORGANISM(S): Mus musculus

SUBMITTER: Riccardo Dalla-Favera 

PROVIDER: E-GEOD-69216 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

The FOXO1 Transcription Factor Instructs the Germinal Center Dark Zone Program.

Dominguez-Sola David D   Kung Jennifer J   Holmes Antony B AB   Wells Victoria A VA   Mo Tongwei T   Basso Katia K   Dalla-Favera Riccardo R  

Immunity 20151124 6


The pathways regulating formation of the germinal center (GC) dark zone (DZ) and light zone (LZ) are unknown. In this study we show that FOXO1 transcription factor expression was restricted to the GC DZ and was required for DZ formation, since its absence in mice led to the loss of DZ gene programs and the formation of LZ-only GCs. FOXO1-negative GC B cells displayed normal somatic hypermutation but defective affinity maturation and class switch recombination. The function of FOXO1 in sustaining  ...[more]

Similar Datasets

2015-12-04 | GSE69216 | GEO
2015-11-23 | E-GEOD-68043 | biostudies-arrayexpress
2017-03-21 | GSE93554 | GEO
2012-09-24 | E-GEOD-38304 | biostudies-arrayexpress
2015-11-23 | GSE68043 | GEO
2015-08-12 | E-GEOD-71295 | biostudies-arrayexpress
2016-08-23 | E-GEOD-80669 | biostudies-arrayexpress
2023-07-06 | GSE212541 | GEO
2023-07-06 | GSE212542 | GEO
2017-03-27 | GSE94733 | GEO