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Expression data from adult wistar female rat 5mm spinal cord tissue

ABSTRACT: We used microarray gene expression analyses to unveil the mechanisms underlying NT-3-chitosan-induced spinal cord regeneration. Complete transaction of thoracic spinal cord at T7/8 was performed, with a 5 mm segment of the spinal cord being removed. The emptied space where either refilled with a 5mm chitosan tube loaded with or without NT-3 (NT-3 or empty tube, ET, respectively) or left alone (lesion control, LC). At 1, 3, 10, 20, 30, 60, 90days post surgery, animals were sacrificed, and 5mm segments of spinal cord at the lesion site, as well as immediately caudal or rostral to the lesion segment were collected labeled with R for rostral, C for caudal, and M for lesion site

ORGANISM(S): Rattus norvegicus

SUBMITTER: Weihong Ge

PROVIDER: E-GEOD-69334 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analyses reveal molecular mechanisms underlying functional recovery after spinal cord injury.

Duan Hongmei H Ge Weihong W Zhang Aifeng A Xi Yue Y Chen Zhihua Z Luo Dandan D Cheng Yin Y Fan Kevin S KS Horvath Steve S Sofroniew Michael V MV Cheng Liming L Yang Zhaoyang Z Sun Yi E YE Li Xiaoguang X

Proceedings of the National Academy of Sciences of the United States of America 20151012 43

Spinal cord injury (SCI) is considered incurable because axonal regeneration in the central nervous system (CNS) is extremely challenging, due to harsh CNS injury environment and weak intrinsic regeneration capability of CNS neurons. We discovered that neurotrophin-3 (NT3)-loaded chitosan provided an excellent microenvironment to facilitate nerve growth, new neurogenesis, and functional recovery of completely transected spinal cord in rats. To acquire mechanistic insight, we conducted a series o ...[more]

PMID: 26460053

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