Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Somatic Primary piRNA Biogenesis Driven by Cis-Acting RNA Elements and Trans- Acting Yb


ABSTRACT: Primary piRNAs in Drosophila ovarian somatic cells arise from piRNA cluster transcripts and the 3′ UTRs of a subset of mRNAs, including Traffic jam (Tj) mRNA. However, it is unclear how these RNAs are determined as primary piRNA sources. Here, we identify a cis-acting 100-nt fragment in the Tj 3′ UTR that is sufficient for producing artificial piRNAs from unintegrated DNA. These artificial piRNAs were effective in endogenous gene transcriptional silencing. Yb, a core component of primary piRNA biogenesis center Yb bodies, directly bound the Tj-cis-element. Disruption of this interaction markedly reduced piRNA production. Thus, Yb is the trans-acting partner of the Tj-cis-element. Yb-CLIP revealed that Yb-binding correlated with somatic piRNA production but Tj-cis-element downstream sequences produced few artificial piRNAs. Thus, Yb determines primary piRNA sources by two modes of action; primary binding to cis-elements to specify substrates, and secondary binding to downstream regions to increase diversity in piRNA populations. HITS-CLIP of Yb in OSCs (Ovarian Somatic Cells) depleted for tj cis-element, and small RNA sequencing of Piwi-piRNAs in OSCs depleted for tj cis-element.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Haruhiko Siomi 

PROVIDER: E-GEOD-69625 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2015-06-05 | GSE69625 | GEO
2014-06-24 | E-GEOD-54875 | biostudies-arrayexpress
2010-10-03 | E-GEOD-23560 | biostudies-arrayexpress
2012-06-30 | E-GEOD-38089 | biostudies-arrayexpress
2019-03-28 | GSE128924 | GEO
2014-06-01 | E-GEOD-52927 | biostudies-arrayexpress
2021-04-16 | PXD019671 | Pride
2021-04-15 | PXD019670 | Pride
2019-04-29 | GSE121605 | GEO
2015-05-22 | E-GEOD-64138 | biostudies-arrayexpress