Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of Brassica rapa leaves elicited with either Pseudomonas syringae pv. maculicola or flg22


ABSTRACT: Phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. Here, we report two new cytochromes P450 from the non-model plant Brassica rapa (Chinese cabbage) that catalyze unprecedented S-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. Our results reveal the first genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites, and have important implications for pathway discovery across >20 recently sequenced non-model crucifers. Leaf mRNA profiles of four conditions (Pseudomonas syringae pv. maculicola, flg22, and their respective mock treatments) were tested in triplicate.

ORGANISM(S): Brassica rapa subsp. pekinensis

SUBMITTER: Elizabeth Sattely 

PROVIDER: E-GEOD-69785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Two cytochromes P450 catalyze S-heterocyclizations in cabbage phytoalexin biosynthesis.

Klein Andrew P AP   Sattely Elizabeth S ES  

Nature chemical biology 20150921 11


Phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. Here, we report two new cytochromes P450 from Brassica rapa (Chinese cabbage) that catalyze unprecedented S-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. Our results provide genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites and have implications for pathway discovery across >20 recent  ...[more]

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