Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse gnotobiotic ileum, listeria infection series

ABSTRACT: Ileal profiles from gnotobiotic mice mono-associated with Listeria species or B. thetaiotaomicron. Samples were derived from 72h colonizations of Fabpi-hEcad transgenic B6 mice fed a standard-chow polysaccharide rich (PR) diet. Experiment Overall Design: Total RNA was prepared from ileum of 72 hour mono-associated gnotobiotic male mice, biotin labeled, and hybridized to 430 2.0 GeneChips.

ORGANISM(S): Mus musculus

SUBMITTER: Justin Sonnenburg 

PROVIDER: E-GEOD-7013 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Functional genomic studies of the intestinal response to a foodborne enteropathogen in a humanized gnotobiotic mouse model.

Lecuit Marc M   Sonnenburg Justin L JL   Cossart Pascale P   Gordon Jeffrey I JI  

The Journal of biological chemistry 20070327 20

Members of the genus Listeria provide a model for defining host responses to invasive foodborne enteropathogens. Active translocation of Listeria monocytogenes across the gut epithelial barrier is mediated by interaction of bacterial internalin (InlA) and its species-specific host receptor, E-cadherin, whereas translocation across Peyer's patches through M-cells is InlA-independent. To define microbial determinants and molecular correlates of the host response to translocation via these two rout  ...[more]

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