Unknown,Transcriptomics,Genomics,Proteomics

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Aspergillus fumigatus in blood reveals a "just wait and see" resting stage behavior


ABSTRACT: Objective: We analyzed changes in A. fumigatus gene expression profile at various stages of an in vitro model of aspergillosis to study the adaptation of A. fumigatus to the blood environment. Results: Most of virulence factors described to be involved in aspergillosis were not activated during the blood phase. We found three active processes to be activated in the later phase that may help to the adaptation: Iron homeostasis, a partial secondary metabolite cluster and the formation of detoxification enzymes. Conclusions: We propose that A. fumigatus is unable to grow in blood and it requires a metabolic change that allows the organism to shut down all uptake and energy-consume mechanisms, resulting in a resting mycelial stage. We performed gene expression profile by sequencing mRNA of A. fumigatus that were growm under two conditions, Minimal Medium (M) and human blood (B), and at different times: before placing the fungus in the final medium (pre), at 30' and at 180', with 2 biological replicates per condition.

ORGANISM(S): Aspergillus fumigatus

SUBMITTER: Sonia Tarazona 

PROVIDER: E-GEOD-70227 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

RNAseq analysis of Aspergillus fumigatus in blood reveals a just wait and see resting stage behavior.

Irmer Henriette H   Tarazona Sonia S   Sasse Christoph C   Olbermann Patrick P   Loeffler Jürgen J   Krappmann Sven S   Conesa Ana A   Braus Gerhard H GH  

BMC genomics 20150827


<h4>Background</h4>Invasive aspergillosis is started after germination of Aspergillus fumigatus conidia that are inhaled by susceptible individuals. Fungal hyphae can grow in the lung through the epithelial tissue and disseminate hematogenously to invade into other organs. Low fungaemia indicates that fungal elements do not reside in the bloodstream for long.<h4>Results</h4>We analyzed whether blood represents a hostile environment to which the physiology of A. fumigatus has to adapt. An in vitr  ...[more]

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