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DNA microarray analysis of PBMCs from BehÃ§etâs disease (BD) patients

ABSTRACT: To examine if gene expression profiles of many BehÃ§etâs disease (BD) patients are commonly abnormal compared to those of healthy volunteers, we performed a genome-wide complementary DNA microarray analysis using an Agilent Hu44K array; RNA samples were isolated from the PBMCs of 41 individual BD patients (32 females and 9 males) and 17 normal volunteers. A total of 41 two color arrays were performed and each individual array included a target sample and a control sample.

ORGANISM(S): Homo sapiens

SUBMITTER: Daisuke Okuzaki

PROVIDER: E-GEOD-70403 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray and whole-exome sequencing analysis of familial Behçet's disease patients.

Okuzaki Daisuke D Yoshizaki Kazuyuki K Tanaka Toshio T Hirano Toru T Fukushima Kohshiro K Washio Takanori T Nojima Hiroshi H

Scientific reports 20160120

Behçet's disease (BD), a chronic systemic inflammatory disorder, is characterized by recurrent oral and genital mucous ulcers, uveitis, and skin lesions. We performed DNA microarray analysis of peripheral blood mononuclear cell (PBMC) mRNA from 41 Japanese BD patients and revealed elevated levels of interleukin (IL) 23 receptor (IL23R) mRNA in many BD patients. DNA sequencing around a SNV (Rs12119179) tightly linked to BD revealed an elevated frequency of the C genotype, consistent with a previo ...[more]

PMID: 26785681

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