Unknown,Transcriptomics,Genomics,Proteomics

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Identification of Arabidopsis thaliana PRR9 regulated clock components and circadian outputs through genome-wide binding sites analysis


ABSTRACT: We identified genome-wide PRR9 targets by conducting chromatin immunoprecipitation followed by high-throughput sequencing. Plants were grown in cycling 12 h light/12 h dark for two weeks before harvesting at ZT4. The four samples include the immunoprecipitated and input control for prr9-1 PRR9::HA-PRR9 CCR2::LUC #109 (HA9) and the prr9-1 (s9c) parental control. PRR5 PRR7 and TOC1 raw data available from the following Series: PRR5: GSE36361 TOC1: GSE35952 PRR7: GSE49282 (GSM1196649 and GSM1196650)

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Tiffany Liu 

PROVIDER: E-GEOD-71397 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A G-Box-Like Motif Is Necessary for Transcriptional Regulation by Circadian Pseudo-Response Regulators in Arabidopsis.

Liu Tiffany L TL   Newton Linsey L   Liu Ming-Jung MJ   Shiu Shin-Han SH   Farré Eva M EM  

Plant physiology 20151119 1


PSEUDO-RESPONSE REGULATORs (PRRs) play overlapping and distinct roles in maintaining circadian rhythms and regulating diverse biological processes, including the photoperiodic control of flowering, growth, and abiotic stress responses. PRRs act as transcriptional repressors and associate with chromatin via their conserved C-terminal CCT (CONSTANS, CONSTANS-like, and TIMING OF CAB EXPRESSION 1 [TOC1/PRR1]) domains by a still-poorly understood mechanism. Here, we identified genome-wide targets of  ...[more]

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