Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sphingobium sp. SYK-6 treated with several carbon sources I

ABSTRACT: Transcriptional profile of RNA extracted from Sphingobium sp. SYK-6 treated with 10 mM dehydrodiconiferyl alcohol (DCA), 10 mM vanillate, or SEMP (10 mM sucrose, 10 mM glutamate, 0.34 mM methionine, and 10 mM proline) comparing labeled SYK-6 genomic DNA. Dual channel experiment, RNA from cells treated with DCA, vanillate, or SEMP vs. genomic DNA. 3 control of genomic DNA, independently grown and harvested.

ORGANISM(S): Sphingobium sp. SYK-6

SUBMITTER: Hirofumi Hara

PROVIDER: E-GEOD-71787 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Sphingobium sp. SYK-6 treated with several carbon sources II

Project description:Transcriptional profile of RNA extracted from Sphingobium sp. SYK-6 treated with 10 mM dehydrodiconiferyl alcohol (DCA), 10 mM vanillate, or SEMP (10 mM sucrose, 10 mM glutamate, 0.34 mM methionine, and 10 mM proline). Dual channel experiment, RNA from cells treated with DCA or vanillate vs SEMP, independently grown and harvested.

2015-08-07 | E-GEOD-71788 | biostudies-arrayexpress

Sphingobium sp. SYK-6 treated with several carbon sources I

2015-08-07 | GSE71787 | GEO

Sphingobium sp. SYK-6 treated with several carbon sources II

2015-08-07 | GSE71788 | GEO

Sphingobium sp. SYK-6 treated with several carbon sources versus SEMP

Project description:Transcriptional profile of RNA extracted from Sphingobium sp. SYK-6 treated with 10 mM pinoresinol (PR), 10 mM acetovanillone (AV), 10 mM phenylcoumarane (PC), 10 mM vanillate (VA), 10 mM vanillin (VN), 10 mM protocatechuate (PCA), 10 mM fellulate (FA), 10 mM 5,5'- dehydrodivanillate (DDVA), 10 mM 1,2‐bis(4‐hydroxy‐3‐methoxyphehyl)‐1,3‐propanediol (B1), 10 mM syringate (SA), 10 mM guaiacylglycerol‐β‐guaiacyl ether (GGE) comparing RNA extracted from SYK-6 treated with SEMP (10 mM sucrose, 10 mM glutamate, 0.34 mM methionine, and 10 mM proline).

2019-07-23 | GSE134094 | GEO

Sphingobium sp. SYK-6 treated with several carbon sources

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-08-07 | E-GEOD-71789 | biostudies-arrayexpress

Sphingobium sp. SYK-6

Project description:Sphingobium sp. SYK-6 genome sequencing

| PRJNA67115 | ENA

Characterisation of p-hydroxycinnamate catabolism in a soil Actinobacterum

Project description:p-Hydroxycinnamates, such as p-coumarate and ferulate, are components of plant cell walls and have a number of commercial applications. Previously, we had shown that the soil Actinobacterium Rhodococcus jostii RHA1 (RHA1) grows on ferulate, catabolizing it via vanillate and the β-ketoadipate pathway. We used transcriptomics to identify genes in RHA1 that were specifically up-regulated during growth on ferulate. These include three operons predicted to encode the uptake and β-oxidative deacetylation of ferulate and p-coumarate: couHLT, couMNO and couR. A couL mutant did not grow on p-coumarate, ferulate or their dihydro derivatives, but grew on vanillate. Purified CouL catalyzed the thioesterification of several p-hydroxycinnamates. Among the tested substrates, the best were p-coumarate and caffeate (kcat/KM ~400 mM-1s-1), and sinapate was not transformed. Of these, p-coumarate was also RHA1’s preferred growth substrate. Although the data indicate that p-hydroxycinnamates are catabolized via β-oxidation, the pathway lacks a typical β-ketothiolase. The data further suggest the involvement of two formaldehyde detoxification pathways in vanillate catabolism. This study augments our understanding of the bacterial catabolism of biomass and facilitates the production of aromatics from renewable feedstocks.

2014-09-28 | GSE56355 | GEO

Sphingobium sp. SYK-6

Project description:Sphingobium sp. SYK-6 treated with several carbon sources I

| PRJNA292087 | ENA

Sphingobium sp. SYK-6

Project description:Sphingobium sp. SYK-6 treated with several carbon sources II

| PRJNA292088 | ENA

Sphingobium Nickel resistance analysis by RNA-seq approach

Project description:Nickel is an essential component of many eukaryotic and prokaryotic metallo-enzymes. Due to its employment in many industrial applications, wastewaters from industrial plants often contain millimolar concentrations of Ni2+ that are toxic and life-threatening for many organism. Several lines of preliminary evidence suggest that members of the genus Sphingobium are able to grow in the presence of high concentrations of metal ions. We have isolated a novel Sphingobium strain (sp. ba1) able to grow in the presence of high concentrations (up to 20 mM) of NiCl2. Sequencing of its genome allowed the identification of several genes coding for proteins potentially involved in efflux-mediated resistance mechanisms. Here we use the RNA-seq approach to analyze the response of the Sphingobium sp. ba1 strain to high concentrations (10 mM) of Ni ions. Transcriptomic data show the differential expression of about one-hundred and twenty genes, most of which are up-regulated and encode proteins such as membrane proteins and components of metal efflux systems, enzymes involved in oxidative stress responses (catalases, peroxidases) and signal transduction systems.

2017-07-03 | E-MTAB-5828 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data