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A polyadenylation factor subunit implicated in regulating oxidative stress responses in Arabidopsis thaliana

ABSTRACT: The oxt6 mutant is an oxidative stress-tolerant Arabidopsis mutant that is deficient in a polyadenylation factor subunit. Expression analysis suggests that impaired poly(A) site choice is responsible for the stress-tolerant phenotype. We used microarrays to understand the link between the polyadenylation defect and stress tolerance. Experiment Overall Design: Arabidopsis plants (the wild-type, oxt6 mutant, and two independent oxt6 transformants each carrying a gene encoding just the smaller of the two At1g30460-encoded proteins) were seeded and grown in soil. Plants were harvested for RNA isolation after 30 days; for this, six samples derived from 10-15 plants were prepared for each line. Ten samples were selected for study â three wild-type, three oxt6, and two from each of the complemented lines. Analysis of independent complemented plants minimizes the possible effects that may arise due to T-DNA insertion.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Arthur Hunt

PROVIDER: E-GEOD-7211 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A polyadenylation factor subunit implicated in regulating oxidative stress responses in Arabidopsis thaliana

Project description:The oxt6 mutant is an oxidative stress-tolerant Arabidopsis mutant that is deficient in a polyadenylation factor subunit. Expression analysis suggests that impaired poly(A) site choice is responsible for the stress-tolerant phenotype. We used microarrays to understand the link between the polyadenylation defect and stress tolerance. Keywords: mutant, wild-type, complemented line comparison

2007-03-07 | GSE7211 | GEO

Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis

Project description:Cytokinin (CK) in plants regulates both developmental processes and adaptation to environmental stress. Arabidopsis histidine phosphotransfer ahp2,3,5 and type-B response regulator arr1,10,12 triple mutants are almost completely defective in CK signaling, and the ahp2,3,5 mutant was reported to be salt tolerant. Here, we demonstrate that the arr1,10,12 mutant is also more tolerant to salt stress than wild-type plants. A comprehensive metabolite profiling coupled with transcriptome analysis of the ahp2,3,5 and arr1,10,12 mutants was conducted to elucidate the salt tolerance mechanisms mediated by CK signaling. Numerous primary (e.g., sugars, amino acids and lipids) and secondary (e.g., flavonoids and sterols) metabolites accumulated in these mutants under both non-saline and saline conditions, suggesting that both pre- and post-stress accumulation of stress-related metabolites contribute to improved salt tolerance in CK-signaling mutants. Specifically, the level of oligosaccharides, amino acids [e.g., proline and gamma-aminobutyric acid], anthocyanins, sterols, and unsaturated triacyglycerols, were higher in the mutant plants than in wild-type plants. Notably, the reprograming of flavonoid and lipid pools was highly coordinated and concomitant with changes in transcriptional levels, indicating that these metabolic pathways are transcriptionally regulated by CK signaling. The discovery of the regulatory role of CK signaling on membrane lipid remodelling provides a greater understanding of CK-mediated salt tolerance in plants. This knowledge will contribute to the development of salt-tolerant crops with the ability to withstand salinity as a key driver to ensure global food security in the era of climate change.

2021-11-25 | MTBLS2532 | MetaboLights

Quantitative proteomics reveals the flooding tolerant mechanism in soybean mutant and ABA treated soybean

Project description:Soybean, which provides abundant proteins and edible oil, is an important crop; however, it is very sensitive to flooding stress. The growth and grain yields of soybean are seriously reduced by flooding stress. To explore the initial flooding-tolerant mechanism of soybean, flooding-tolerant mutant line and ABA treated soybean were used. Because both material and treatment showed the flooding tolerance, the commonly changed proteins in plants under initial flooding stress were identified. To identify the common proteins, gel free/label free proteomic technique was used.

2019-01-09 | PXD003329 | Pride

Expression data in response to abiotic stresses in tomato at flowering stage

Project description:A comparative study ware made to know the abiotic stress tolerance machanism between tolerant and susceptible plants at flowering stage. The tolerance in response to abiotic stresses are sum of expression of thousands of genes at a particular stage. Tomato plants were exposed to drought and heat stress for RNA extraction and hybridization on Affymetrix microarrays. To study the molecular mechanism of abiotic stress tolerance to increase the tolerance in tomato plants, transcripts of tolerant and susceptible plants at flowering stage were compared in response to heat and water stress.

2010-06-23 | E-GEOD-22304 | biostudies-arrayexpress

Analysis of a N-6 adenosine methylation deficient plant

Project description:The embryo lethal adenosine methylase tDNA knockout line SALK_074069 was partially complemented with its cDNA driven by the embryo specific ABI3 promoter (A6 lines). The plants have reduced adenosine methylation and show pleiotropic phenotypes. Rosette leaves were harvested from 3 week old plants, both wild-type and mutant plants in triplicate and analysed using the Affymetrix ATH1 array.

2012-05-02 | GSE34924 | GEO

au08-06_mpk6_heat_stressed-Transcriptomic analysis of mpk6 KO mutant after heat stress treatment

Project description:au08-06_mpk6_heat_stressed - au08-06_mpk6_heat_stressed - Mitogen Activated Protein Kinase (MAPK) signaling pathways are key regulators of cell proliferation, differentiation and stress effectors. The core of the MAP kinase signal transduction cascade is composed of a three-kinase module consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK). The signaling pathway is activated upon stimulation by a phosphorylation cascade. In previous studies, it was shown that the mpk6 KO mutant plants are significantly more tolerant to heat stress in comparison to wt and that after 3h treatment at 37°C, an activation of heat-shock proteins occures in the mpk6 mutant. To better understand the changes occuring in the mpk6 mutant upon heat stress at the gene expression level, we would like to perform a microarray transcriptomic analysis. - Mitogen Activated Protein Kinase (MAPK) signaling pathways are key regulators of cell proliferation, differentiation and stress effectors. The core of the MAP kinase signal transduction cascade is composed of a three-kinase module consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK). The signaling pathway is activated upon stimulation by a phosphorylation cascade. In previous studies, it was shown that the mpk6 KO mutant plants are significantly more tolerant to heat stress in comparison to wt and that after 3h treatment at 37°C, an activation of heat-shock proteins occures in the mpk6 mutant. To better understand the changes occuring in the mpk6 mutant upon heat stress at the gene expression level, we would like to perform a microarray transcriptomic analysis. Keywords: treated vs untreated comparison

2010-06-29 | GSE22568 | GEO

ß-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells

Project description:In plants, drought stress is a major growth limiting factor causing cell water loss through open stomata. In this study, guard cell-specific transcripts from drought-stressed Arabidopsis plants were analyzed and a down-regulation of β-amylase 1 (BAM1) was found. In previous studies, BAM1 was shown to be involved in stomatal starch degradation under ambient conditions. Impaired starch breakdown of bam1 mutant plants was accompanied by decreased stomatal opening. Here, we show that drought tolerance of bam1 mutant plants is improved as compared to wild type controls. Microarray-analysis of stomata-specific transcripts from bam1 mutant plants revealed a significant down-regulation of genes encoding aquaporins, auxin- and ethylene-responsive factors and cell-wall modifying enzymes. This expression pattern suggests that reduced water-uptake and limited cell wall extension are associated with the closed state of stomata of bam1 mutant plants. Together these data suggest that regulation of stomata-specific starch turnover is important for adapting stomata opening to environmental needs and its breeding manipulation may result in drought tolerant crop plants. Stress induced gene expression in Arabidopsis leaves and Stomata was measured after exposure to single drought stress. Drought stress conditions were analysed for both, Col-0 plants and a T-DNA insertion line for β-amylase 1. Six week old plants were treated with drought stress (5 days) according to Prasch and Sonnewald, 2013. Two to three biological replicates have been hybridized for each treatment.

2015-06-30 | E-GEOD-59315 | biostudies-arrayexpress

ß-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells

2015-06-30 | GSE59315 | GEO

ß-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells

2015-06-30 | GSE66105 | GEO

ß-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells

Project description:In plants, drought stress is a major growth limiting factor causing cell water loss through open stomata. In this study, guard cell-specific transcripts from drought-stressed Arabidopsis plants were analyzed and a down-regulation of β-amylase 1 (BAM1) was found. In previous studies, BAM1 was shown to be involved in stomatal starch degradation under ambient conditions. Impaired starch breakdown of bam1 mutant plants was accompanied by decreased stomatal opening. Here, we show that drought tolerance of bam1 mutant plants is improved as compared to wild type controls. Microarray-analysis of stomata-specific transcripts from bam1 mutant plants revealed a significant down-regulation of genes encoding aquaporins, auxin- and ethylene-responsive factors and cell-wall modifying enzymes. This expression pattern suggests that reduced water-uptake and limited cell wall extension are associated with the closed state of stomata of bam1 mutant plants. Together these data suggest that regulation of stomata-specific starch turnover is important for adapting stomata opening to environmental needs and its breeding manipulation may result in drought tolerant crop plants. Stress induced gene expression in Arabidopsis stomata was measured after exposure to single heat stress. Heat stress conditions were analyzed for both Col-0 plants and a T-DNA insertion line for β-amylase 1. Three days before harvesting heat stress was applied (32°C/28°C). Samples were taken by pooling the stomata of six to eight leaves per sample.

2015-06-30 | E-GEOD-66105 | biostudies-arrayexpress

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