Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

A polyadenylation factor subunit implicated in regulating oxidative stress responses in Arabidopsis thaliana


ABSTRACT: The oxt6 mutant is an oxidative stress-tolerant Arabidopsis mutant that is deficient in a polyadenylation factor subunit. Expression analysis suggests that impaired poly(A) site choice is responsible for the stress-tolerant phenotype. We used microarrays to understand the link between the polyadenylation defect and stress tolerance. Experiment Overall Design: Arabidopsis plants (the wild-type, oxt6 mutant, and two independent oxt6 transformants each carrying a gene encoding just the smaller of the two At1g30460-encoded proteins) were seeded and grown in soil. Plants were harvested for RNA isolation after 30 days; for this, six samples derived from 10-15 plants were prepared for each line. Ten samples were selected for study – three wild-type, three oxt6, and two from each of the complemented lines. Analysis of independent complemented plants minimizes the possible effects that may arise due to T-DNA insertion.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Arthur Hunt 

PROVIDER: E-GEOD-7211 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2007-03-07 | GSE7211 | GEO
2021-11-25 | MTBLS2532 | MetaboLights
2019-01-09 | PXD003329 | Pride
2010-06-23 | E-GEOD-22304 | biostudies-arrayexpress
2012-05-02 | GSE34924 | GEO
2010-06-29 | GSE22568 | GEO
2015-06-30 | E-GEOD-59315 | biostudies-arrayexpress
2015-06-30 | GSE59315 | GEO
2015-06-30 | GSE66105 | GEO
2015-06-30 | E-GEOD-66105 | biostudies-arrayexpress