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Biological function of antisense RNAs that are synthesized by RNA-dependent RNA polymerases under abiotic stress

ABSTRACT: To identify RDR1/2/6-dependent antisense RNA loci under 2hr drought stress, expression profiles of Poly (A+) RNA and Poly (A-) RNA between rdr1/2/6 and wild-type using the custom microarray (GPL19830) were analyzed. rdr1/2/6 and wild-type plants (Arabidopsis thaliana ecotype Columbia) were grown on MS medium for 2 hours. Then, the plants were transferred to plastic dish and kept for 1 week. Poly (A+) RNA and Poly (A-) fractions were separated from 100Î¼g total RNA by treating with Ambion Poly A purist twice. Poly (A-) RNA sample was prepared by depleting rRNA using Invitrogen RiboMinus Plant Kit. 5 ng of Poly (A+) RNA and 1.4 Î¼g of Poly (A-) RNA samples were used for the expression profiling using the custom array (GPL19830).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Akihiro Matsui

PROVIDER: E-GEOD-72309 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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