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Transcription profiling of normal mouse lung from age matched untreated controls from AJ mice were from two time points 24 to 26 weeks (N) or 42 weeks (N42) after saline injection

ABSTRACT: AJ mouse is susceptible to lung carcinogenesis from urethane treatment and is a good model for human adenocarcinoma. We completed a study using microarray analysis to identify a unique macrophage expression signature in the lung tumor microenvironment. Experiment Overall Design: RNA from lung tissues of age matched untreated controls (Normal) from AJ mice were from two time points 24 to 26 weeks (N) or 42 weeks (N42) after saline injection. Datasets were compared to previously published adjacent to tumor lung tissues in Stearman et al Am J Path 167 1763 (2005).

ORGANISM(S): Mus musculus

SUBMITTER: Robert Stearman

PROVIDER: E-GEOD-7244 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A macrophage gene expression signature defines a field effect in the lung tumor microenvironment.

Stearman Robert S RS Dwyer-Nield Lori L Grady Michael C MC Malkinson Alvin M AM Geraci Mark W MW

Cancer research 20080101 1

One area of intensive investigation is to understand complex cellular and signaling interactions in the tumor microenvironment. Using a novel, although straightforward, microarray approach, we defined a gene expression signature from the lung tumor microenvironment in the murine A/J-urethane model of human lung adenocarcinoma. The tumor microenvironment is reflected by the composition of the cell types present and alterations in mRNA levels, resulting in a "Field Effect" around the tumor. The ge ...[more]

PMID: 18172294

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