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Differential expression of GFP+ and RFP+ TriPyMT cells

ABSTRACT: Tri-PyMT cell is a breast tumor cell line established from Tri-PyMT (MMTV-PyMT/fsp1-Cre/Rosa26-RGFP) primary breast tumors. The cells switch from RFP+ to GFP+ during epithelial to mesenchymal transition (EMT). Differential expressed genes between EMT and non-EMT tumor cells will reveal interesting targets for anti-EMT approaches. Tri-PyMT cells were culture in medium with serum (10%FBS). Cells were treated with or without 4-hydroperoxy-cyclophosphamide (4µM) for 7 days and sorted by flow cytometry into RFP+ and GFP+ subpopulations for RNA extraction and subsequent RNA-Seq analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Dingcheng Gao

PROVIDER: E-GEOD-72480 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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