Transcriptional Response of Mycobacterium tuberculosis to Growth without Detergent
Ontology highlight
ABSTRACT:
ORGANISM(S): Mycobacterium tuberculosis
SUBMITTER: Brian Weinrick
PROVIDER: E-GEOD-72745 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
The Type of Growth Medium Affects the Presence of a Mycobacterial Capsule and Is Associated With Differences in Protective Efficacy of BCG Vaccination Against Mycobacterium tuberculosis.
Prados-Rosales Rafael R Carreño Leandro J LJ Weinrick Brian B Batista-Gonzalez Ana A Glatman-Freedman Aarona A Xu Jiayong J Chan John J Jacobs William R WR Porcelli Steven A SA Casadevall Arturo A
The Journal of infectious diseases 20160418 3
<h4>Background</h4>Bacillus Calmette-Guerin (BCG) vaccine is widely used for the prevention of tuberculosis, despite limited efficacy. Most immunological studies of BCG or Mycobacterium tuberculosis strains grow bacteria in the presence of detergent, which also strips the mycobacterial capsule. The impact of the capsule on vaccine efficacy has not been explored.<h4>Methods</h4>We tested the influence of detergent in cultures of BCG and M. tuberculosis strains on the outcome of vaccination experi ...[more]
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