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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic and Epigenetic analysis of cell line derivatives from H2087 and HCC1954 cell lines

ABSTRACT: We profiled the transcriptomes of latency-competent cells derived from the human cancer cell lines H2087 (lung adenocarcinoma) and HCC1954 (breast adenocarcinoma) in mitogen-rich and mitogen-low media (MRM and MLM, respectively). In addition, we analyzed the epigenetic landscape of these cell lines under MLM conditions. H2087 and HCC1954 parental and latency-competent cell derivatives (LCCs) were grown for 48hr in mitogen-rich or mitogen-low conditions in vitro, and whole RNA was extracted for RNA-seq profiling. Cell lines were also grown in MLM conditions and DNA extracted for ChIP-Seq experiments.

ORGANISM(S): Homo sapiens

SUBMITTER: Danilo Macalinao

PROVIDER: E-GEOD-72956 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Metastatic Latency and Immune Evasion through Autocrine Inhibition of WNT.

Malladi Srinivas S Macalinao Danilo G DG Jin Xin X He Lan L Basnet Harihar H Zou Yilong Y de Stanchina Elisa E Massagué Joan J

Cell 20160301 1

Metastasis frequently develops years after the removal of a primary tumor, from a minority of disseminated cancer cells that survived as latent entities through unknown mechanisms. We isolated latency competent cancer (LCC) cells from early stage human lung and breast carcinoma cell lines and defined the mechanisms that suppress outgrowth, support long-term survival, and maintain tumor-initiating potential in these cells during the latent metastasis stage. LCC cells show stem-cell-like character ...[more]

PMID: 27015306

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