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Gene expression signatures in cell cycle sorted cells [HeLa_mRNA_array]

ABSTRACT: For the further examination of cell cycle dependent gene expression profile, DNA content based fluorescence activated cell sorting (cell cycle sort) was performed on human transformed cancer cell lines and primary, untransformed fibroblasts. Phase-dependent gene expression profiling was performed on G1, S and G2 phases. Results were validated by quantitative real-time PCR. Phase-dependent gene expression profiling was performed on sorted human cancer (cervical - HeLa and adrenocortical - NCI-H295R) cells and primary untransformed fibroblasts (HDFa). G1, S and G2 phases were successfully sorted and analyzed. HeLa_mRNA dataset

ORGANISM(S): Homo sapiens

SUBMITTER: Vince Grolmusz 

PROVIDER: E-GEOD-73196 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Fluorescence activated cell sorting followed by small RNA sequencing reveals stable microRNA expression during cell cycle progression.

Grolmusz Vince Kornél VK   Tóth Eszter Angéla EA   Baghy Kornélia K   Likó István I   Darvasi Ottó O   Kovalszky Ilona I   Matkó János J   Rácz Károly K   Patócs Attila A  

BMC genomics 20160527

<h4>Background</h4>Previously, drug-based synchronization procedures were used for characterizing the cell cycle dependent transcriptional program. However, these synchronization methods result in growth imbalance and alteration of the cell cycle machinery. DNA content-based fluorescence activated cell sorting (FACS) is able to sort the different cell cycle phases without perturbing the cell cycle. MiRNAs are key transcriptional regulators of the cell cycle, however, their expression dynamics du  ...[more]

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