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FOXM1 Cistrome Predicts Breast Cancer Metastatic Outcome Better Than FOXM1 Expression Levels or Tumor Proliferation Index

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Christina Yau

PROVIDER: E-GEOD-73371 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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FOXM1 cistrome predicts breast cancer metastatic outcome better than FOXM1 expression levels or tumor proliferation index.

Yau Christina C Meyer Laurence L Benz Stephen S Vaske Charles C Scott Gary G Egan Brian B Labhart Paul P Pourmand Nader N Benz Christopher C CC

Breast cancer research and treatment 20151012 1

FOXM1 is a key transcription factor regulating cell cycle progression, DNA damage response, and a host of other hallmark cancer features, but the role of the FOXM1 cistrome in driving estrogen receptor-positive (ER+) versus estrogen receptor-negative (ER-) breast cancer clinical outcomes remains undefined. Chromatin immunoprecipitation sequencing (ChIP-Seq) coupled with RNA sequencing (RNA-Seq) analyses was used to identify FOXM1 target genes in breast cancer cells (MCF-7) where FOXM1 expression ...[more]

PMID: 26456572

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FOXM1 Cistrome Predicts Breast Cancer Metastatic Outcome Better Than FOXM1 Expression Levels or Tumor Proliferation Index [ChIP-Seq]

Project description:FOXM1 is a key transcription factor regulating cell cycle progression, DNA damage response, and a host of other hallmark cancer features, but the role of the FOXM1 cistrome in driving estrogen receptor-positive (ER+) vs. ER- breast cancer clinical outcomes remains undefined. Chromatin immunoprecipitation sequencing (ChIP-Seq) coupled with RNA sequencing (RNA-Seq) analyses was used to identify FOXM1 target genes in breast cancer cells (MCF-7) where FOXM1 expression was either induced by cell proliferation or repressed by p53 upregulation.

2015-10-26 | E-GEOD-72977 | biostudies-arrayexpress

FOXM1 Cistrome Predicts Breast Cancer Metastatic Outcome Better Than FOXM1 Expression Levels or Tumor Proliferation Index [RNA-Seq]

2015-10-26 | E-GEOD-73370 | biostudies-arrayexpress

Inhibition of PARP14 restores response to α-PD1 immune checkpoint inhibition in tumours with resistance driven by IFNγ. PARP14 study.

Project description:While immune checkpoint blockade therapy (ICBT) benefits cancer patients, many fail to maintain their response due to adaptive resistance mechanisms. IFNγ is critical for cellular immunity, but also promotes adaptive resistance to ICBT. We have established a role for PARP14 in mediating IFNγ-induced adaptive resistance. We confirm that chronic pre-treatment of tumour cells with IFNγ confers resistance to α-PD-1 antibodies in syngeneic mouse tumour models. We identified that PARP14 was consistently upregulated in cancer cells treated chronically with IFNγ as well as in IFNγ-high melanoma samples. Further, we showed that PARP14 knockdown or pharmacological inhibition restores sensitivity to α-PD-1 antibodies accompanied by increased immune cell infiltration into tumours but the decreased presence of regulatory T cells. RNA sequencing analysis of tumours and cultured cells treated with PARP14 inhibitor showed an upregulation of inflammatory-related pathways. In conclusion, PARP14 is an actionable target for reversing IFNγ-driven adaptive resistance to ICBT.

2023-08-08 | E-MTAB-12195 | biostudies-arrayexpress

Early Response Genes in Osteoblasts Exposed to Dynamic Fluid Flow

Project description:Cyclic mechanical loads applied to the skeleton increase bone formation. Dynamic fluid flow is a potent anabolic stimulus for cultured osteoblasts. In this study, gene profiles involved in mediating the anabolic response of MC3T3 osteoblasts to dynamic fluid flow are investigated. MC3T3 osteoblast RNA was harvested 30-minutes and 1-hour post-stimulation respectively as experimental samples for comparison to the control group without dynamic fluid flow.

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Transcription profiling of S. cerevisiae oxidative stress response

Project description:Oxidative stress is a harmful condition in a cell, tissue, or organ, caused by an imbalnace between reactive oxygen species and other oxidants and the capacity of antioxidant defense systems to remove them. The budding yeast S. cerevisiae has been the major eukaryotic model for studies of response to oxidative stress. We used microarrays to study the genome-wide temporal response of the yeast S. cerevisiae to oxidative stress induced by cumene hydroperoxide. Experiment Overall Design: The effects of oxidative stress induced by CHP on the transcriptional profile of S. cerevisiae was studied from a dynamical perspective. Yeast cultures were grown in controlled batch conditions, in 1 L fermentors. Three replicate cultures in mid-exponential phase were exposed to 0.19 mM CHP, while three non-treated cultures were used as controls. Samples were collected at t=0 (immediately before adding CHP) and at 3,6,12,20,40,70 and 120 min after adding the oxidant. Samples were processed for RNA extraction and profiled using Affymetrix Yeast Genome S98 arrays.

2008-06-15 | E-GEOD-7645 | biostudies-arrayexpress

Expression data for Saccharomyces cerevisiae oxidative stress response

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2013-12-17 | E-GEOD-26169 | biostudies-arrayexpress

Expression data from human carcinoma (MCF7) derived cells that have been exposed to insulin analogues

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2015-01-29 | E-GEOD-65398 | biostudies-arrayexpress

Transcription profiling of human response to carboplatin in chemosensitive and chemoresistant ovarian cancer cells.

Project description:Use of expression arrays to identify genes from ovarian cancer cells that are associated with carboplatin sensitivity and resistance as defined by ChemoFX assay.

2007-09-01 | E-GEOD-1926 | biostudies-arrayexpress

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2021-01-04 | PXD021575 | Pride

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