Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human pre-malignant breast cancer samples


ABSTRACT: Enlargement of normal terminal duct lobular units (TDLUs) by hyperplastic columnar epithelial cells is one of the most common abnormalities of growth in the adult female human breast. These hyperplastic enlarged lobular units (HELUs) are important clinically as the earliest histologically identifiable potential precursor of breast cancer. The causes of the hyperplasia are unknown but may include estrogen-simulated growth mediated by estrogen receptor alpha, which is highly elevated in HELUs and may be fundamental to their development. This study used DNA microarray technology and RNA from microdissected pure epithelial cells to learn more about changes in gene expression and molecular pathways associated with the development of HELUs from TDLUs Experiment Overall Design: Samples for the microarray studies were derived from 8 formalin-fixed paraffin-embedded tissue (FFPET) biopsies containing paired normal TDLUs and well-developed HELUs from non-cancerous adult female human breasts obtained within the past 3 years. Populations of nearly pure (>95%) epithelial cells were isolated from the TDLUs and HELUs in each biopsy (approximately 25,000 cells/sample) by laser capture microdissection (LCM)

ORGANISM(S): Homo sapiens

SUBMITTER: Sangjun Lee 

PROVIDER: E-GEOD-7377 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Alterations of gene expression in the development of early hyperplastic precursors of breast cancer.

Lee Sangjun S   Medina Dan D   Tsimelzon Anna A   Mohsin Syed K SK   Mao Sufeng S   Wu Yun Y   Allred D Craig DC  

The American journal of pathology 20070701 1


Enlargement of normal terminal duct lobular units (TDLUs) by hyperplastic columnar epithelial cells is one of the most common abnormalities of growth in the adult female human breast. These hyperplastic enlarged lobular units (HELUs) are important clinically as the earliest histologically identifiable potential precursor of breast cancer. The causes of the hyperplasia are unknown but may include estrogen-simulated growth mediated by estrogen receptor-alpha, which is highly elevated in HELUs and  ...[more]

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