Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

39 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of E. coli covering four phases of growth in Fis KO and WT (Fis+) strains

ABSTRACT: This SuperSeries is composed of the following subset Series:; GSE7379: Fis KO strain; GSE7380: WT (Fis+) strain Experiment Overall Design: Refer to individual Series

ORGANISM(S): Escherichia coli

SUBMITTER: Robert Osuna

PROVIDER: E-GEOD-7398 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Effects of Fis on Escherichia coli gene expression during different growth stages.

Bradley Meranda D MD Beach Michael B MB de Koning A P Jason APJ Pratt Timothy S TS Osuna Robert R

Microbiology (Reading, England) 20070901 Pt 9

Fis is a nucleoid-associated protein in Escherichia coli that is abundant during early exponential growth in rich medium but is in short supply during stationary phase. Its role as a transcriptional regulator has been demonstrated for an increasing number of genes. In order to gain insight into the global effects of Fis on E. coli gene expression during different stages of growth in rich medium, DNA microarray analyses were conducted in fis and wild-type strains during early, mid-, late-exponent ...[more]

PMID: 17768236

Similar Datasets

Transcription profiling of E.coli wild type (Fis+) strain

Project description:Fis is a nucleoid-associated protein in E. coli that is abundant during early logarithmic growth in rich medium but is in short supply during stationary phase. Its role as a transcriptional regulator has been demonstrated for an increasing number of genes. In order to gain insight into the global effects of Fis on E. coli gene expression during different stages of growth in rich medium, DNA microarray analyses were conducted in fis and wild type strains during early log, mid log, late log, and stationary growth phases. We used microarrays to detail the global impact of Fis on gene expression in Escherichia coli Experiment Overall Design: E.coli cells were were grown and samples were taken at different times during growth: 90 min (early logarithmic phase), 150 min (mid-logarithmic phase), 240 min (late logarithmic phase), and 360 min (early stationary phase) for RNA extraction and hybridization on Affymetrix microarrays. This was done in triplicate and the raw data was analyzed using Microarray Analysis Suite version 5.0 (Affymetrix).

2008-06-15 | E-GEOD-7380 | biostudies-arrayexpress

Transcription profiling of E.coli Fis knockout strain

2008-06-15 | E-GEOD-7379 | biostudies-arrayexpress

WT (Fis+) strain

2007-08-15 | GSE7380 | GEO

Fis KO strain

2007-08-15 | GSE7379 | GEO

CRP and FIS

Project description:CRP and FIS

| PRJNA412865 | ENA

Fis occupancy along the Salmonella enterica serovar Typhimurium SL1344 fis::3XFlag strain grown in different aeration regimes.

Project description:The regulatory role of the Fis protein in fis and in the transcription of several gene regions during mid-exponential and late-stationary phase, and during different growth aeration regimes, has been investigated. Studies were done during those two growth phases and in aerated and non-aerated (microaerobic) conditions, to measure Fis enrichment and binding peaks in strategic gene regions by genome-wide microarray analysis ChIP-chip. This research investigation points to central roles for SPI-1, SPI-2, DNA gyrase and topoisomerase I, the elements of the stringent response, and the regulatory function of Fis-binding patterns, in setting and re-setting the activity of the fis gene and other involved promoters as a function of the growth conditions and aeration regimes experienced by Salmonella. One sample with four different treatments. Three biological replicates per sample. The Aerated 2 hour sample was set as the control condition for all samples.

2013-05-01 | E-GEOD-38661 | biostudies-arrayexpress

Fis occupancy along the Salmonella enterica serovar Typhimurium SL1344 fis::3XFlag strain grown in different aeration regimes.

2013-05-01 | GSE38661 | GEO

Transcription profiling by array of E. coli MG1655 strains with deletions of fis and hns in early-exponential, mid-exponential, transition-to-stationary and stationary phases

Project description:Differential expression of genes in E. coli MG1655 strains with deletions of fis and hns was assessed under early-exponential, mid-exponential, transition-to-stationary and stationary phases of growth in LB medium.

2010-09-01 | E-MEXP-2838 | biostudies-arrayexpress

Aeromonas jandaei DSM 7311 T6SS secretome - comparative proteomics

Project description:Label-free quantification comparative proteomics analysis of proteins secreted to the media by the T6SS of Aeromonas jandaei DSM 7311

2024-08-13 | PXD054809 | Pride

The evolutionary impact of Intragenic FliA Promoters

Project description:We looked at binding sites in a FliA FLAG-tagged wild-type Salmonella enterica serovar Typhimurium strain.

2017-09-18 | E-MTAB-6049 | biostudies-arrayexpress