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Oocyte-expressed Yes-associated Protein (YAP) is a Key Activator of the Early Zygotic Genome in Mouse

ABSTRACT: We generated maternal and paternal mouse models with Yap1-deletion, and elucidated the function of maternal YAP in zygotic genome activation. RNA sequencing with 2 WT oocyte samples at GV stage, 2 YAP-/- oocyte samples at GV stage, 1 WT embryo samples at 4cell stage, 1 YAP-/- embryo samples at at 4-cell stage.

ORGANISM(S): Mus musculus

SUBMITTER: yujiao dang

PROVIDER: E-GEOD-74344 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Oocyte-expressed yes-associated protein is a key activator of the early zygotic genome in mouse.

Yu Chao C Ji Shu-Yan SY Dang Yu-Jiao YJ Sha Qian-Qian QQ Yuan Yi-Feng YF Zhou Jian-Jie JJ Yan Li-Ying LY Qiao Jie J Tang Fuchou F Fan Heng-Yu HY

Cell research 20160223 3

In early mammalian embryos, the genome is transcriptionally quiescent until the zygotic genome activation (ZGA) which occurs 2-3 days after fertilization. Despite a long-standing effort, maternal transcription factors regulating this crucial developmental event remain largely elusive. Here, using maternal and paternal mouse models of Yap1 deletion, we show that maternally accumulated yes-associated protein (YAP) in oocyte is essential for ZGA. Maternal Yap1-knockout embryos exhibit a prolonged t ...[more]

PMID: 26902285

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