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Serum Conditions Do Not Abolish The Tumorigenicity of Glioma Stem Cells

ABSTRACT: The subset of GBM patient samples gives rise to adherent cultures even in sphere culture conditions. Most samples in this subset are tumorigenic and exhibit a hybrid expression profile when tested with the marker panel. Cultures from these samples have a predominantly mesenchymal character based on substrate adherence, morphology, differentiation potential and gene expression. Total RNA isolated from glioblastoma stem cells (GSC) cultured as spheres was compared to that from adherent GSCs cultured in sphere culture conditions that exhibited both GSC and mesenchymal properties.

ORGANISM(S): Homo sapiens

SUBMITTER: Biljana Stangeland

PROVIDER: E-GEOD-74571 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential propagation of stroma and cancer stem cells dictates tumorigenesis and multipotency.

Behnan J J Stangeland B B Hosainey S A M SA Joel M M Olsen T K TK Micci F F Glover J C JC Isakson P P Brinchmann J E JE

Oncogene 20160627 4

Glioblastoma Multiforme (GBM) is characterized by high cancer cell heterogeneity and the presence of a complex tumor microenvironment. Those factors are a key obstacle for the treatment of this tumor type. To model the disease in mice, the current strategy is to grow GBM cells in serum-free non-adherent condition, which maintains their tumor-initiating potential. However, the so-generated tumors are histologically different from the one of origin. In this work, we performed high-throughput marke ...[more]

PMID: 27345406

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