Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street? [Affymetrix]

ABSTRACT: The objective of this study was to examine the effect of the presence of a single or multiple embryo(s) on the transcriptome of the bovine oviduct. In Experiment 1, cyclic (non-bred, n = 6) and pregnant (artificially inseminated, n = 11) heifers were slaughtered on Day 3 after estrus, and the ampulla and isthmic regions of the oviduct ipsilateral to the corpus luteum were separately flushed. Oviductal epithelial cells from the isthmus region, in which all oocytes/embryos were located, were snap-frozen for microarray analysis. In Experiment 2, heifers were divided into cyclic (non-bred, n = 6) or pregnant (multiple embryo transfer, n = 10) groups. In vitro-produced presumptive zygotes were transferred endoscopically to the ipsilateral oviduct on Day 1.5 post estrus (n = 50 zygotes per heifer). Heifers were slaughtered on Day 3 and oviductal isthmus epithelial cells were recovered for RNA sequencing. Microarray analysis in Experiment 1 failed to detect any difference in the transcriptome of the oviductal isthmus induced by the presence of a single embryo. In Experiment 2, following multiple embryo transfer, RNA sequencing revealed 278 differentially expressed genes of which 123 were up- and 155 were down-regulated in pregnant heifers. Most of the down-regulated genes were related to immune function. Five samples from pregnant heifers and five control, cyclic heifers were analysed

ORGANISM(S): Bos taurus

SUBMITTER: Peadar O'Gaora

PROVIDER: E-GEOD-74593 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?

Maillo Veronica V Gaora Peadar Ó PÓ Forde Niamh N Besenfelder Urban U Havlicek Vitezslav V Burns Gregory W GW Spencer Thomas E TE Gutierrez-Adan Alfonso A Lonergan Patrick P Rizos Dimitrios D

Biology of reproduction 20150429 6

This study examined the effect of the presence of single or multiple embryos on the transcriptome of the bovine oviduct. In experiment 1, cyclic (nonbred, n = 6) and pregnant (artificially inseminated, n = 11) heifers were slaughtered on Day 3 after estrus, and the ampulla and isthmic regions of the oviduct ipsilateral to the corpus luteum were separately flushed. Oviductal epithelial cells from the isthmus region, in which all oocytes/embryos were located, were snap-frozen for microarray analys ...[more]

PMID: 25926440

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Project description:The study of early maternal-embryonic cross-talk remains one of the most challenging topics in reproductive biology. Understanding the physiological mechanisms involved in the interactions between the maternal reproductive tract and the developing embryo is essential for enhancing bovine reproductive efficiency. This complex communication starts within the oviduct, where the modulation of biological processes important for ensuring embryo quality is partially facilitated through extracellular vesicles (EVs). Utilizing a combination of in vivo and in vitro models this study had three main objectives: (i) to examine the protein cargo of EVs isolated from the oviductal fluid (OF) of cyclic and pregnant heifers to understand their role in maternal-embryonic communication in vivo; (ii) to characterize the protein profile of EVs in conditioned medium (CM) resulting from the culture of oviductal explants alone (Exp) or in the presence of 8- to 16-cell stage embryos (Exp+Emb); and (iii) to compare the protein cargo of EVs from Exp with EVs from cyclic heifers and EVs from Exp+Emb with EVs from pregnant heifers. Proteins were considered ‘present’ if detected in at least three out of five replicates and considered ‘exclusive’ if detected in at least three out of five replicates within one group but absent in all samples of other groups. We identified 670 and 1508 proteins in the OF-EVs of cyclic and pregnant heifers, respectively. Among these, 651 proteins were found in both groups, while 42 were exclusive to OF-EVs from the pregnant group. Within the 651 proteins detected in both groups, 32 were identified as differently abundant proteins (DAPs). In pregnant heifers, DAPs were mainly related to genome activation, DNA repair, embryonic cell differentiation, migration, and immune tolerance. In vitro, we identified 859 proteins in the CM-EVs from Exp alone, 627 from Exp+Emb, and 111 in the CM-EVs from Emb alone. In the qualitative analysis between the three groups, 81 proteins were commonly detected, 485 were common to Exp and Exp+Emb, 17 were common to Exp and Emb, 5 were common to Exp+Emb and Emb, 5 were unique to Exp, 6 were unique to Exp+Emb, and none were unique to Emb. Proteins identified when there is an interaction between the oviduct and the embryo in vitro, corresponding to Exp+Emb group, were associated with immune tolerance, structural activity, binding, and cytoskeletal regulation. In vivo and in vitro EVs exhibit distinct qualitative and quantitative protein contents, both when comparing EVs produced in the absence of an embryo (Cyclic and Exp) and those that have undergone embryo-oviduct interaction (Pregnant and Exp+Emb). The observed changes in the protein cargo of EVs due to maternal-embryonic communication in vitro and in vitro suggest that in vivo, the interaction between the embryo and the maternal milieu initiates within the oviduct and is potentially facilitated by EVs and their protein contents.

Dataset Information

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street? [Affymetrix]

Publications

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?

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