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Analysis of Fusarium verticillioides gene expression in response to 2-benzoxazolinone (BOA)

ABSTRACT: Investigation of whole genome gene expression level changes in F. verticillioides FRC M-3125 when exposed to 50 ug/ml 2-benzoxazolinone (BOA). Cultures were harvested two hours after exposure. Assessed in reference to control cultures of M-3125 exposed to ethanol (1% final concentration) since BOA was dissolved in ethanol. A four chip study using total RNA recovered from two separate control cultures of F. verticillioides FRC M-3125 in PDB containing 1% ethanol and two separate experimental cultures of M-3125 grown in PDB plus the added treatment of 50 ug/ml ethanolic BOA. The cultures were incubated for two hours on a rotary shaker (200 rpm) at 27C in the dark and then harvested by vacuum filtration and frozen in liquid nitrogen. The chips were a F. verticillioides custom 14K array based on platform GPL14669.

ORGANISM(S): Fusarium verticillioides

SUBMITTER: Anthony Glenn

PROVIDER: E-GEOD-74832 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Two Horizontally Transferred Xenobiotic Resistance Gene Clusters Associated with Detoxification of Benzoxazolinones by Fusarium Species.

Glenn Anthony E AE Davis C Britton CB Gao Minglu M Gold Scott E SE Mitchell Trevor R TR Proctor Robert H RH Stewart Jane E JE Snook Maurice E ME

PloS one 20160125 1

Microbes encounter a broad spectrum of antimicrobial compounds in their environments and often possess metabolic strategies to detoxify such xenobiotics. We have previously shown that Fusarium verticillioides, a fungal pathogen of maize known for its production of fumonisin mycotoxins, possesses two unlinked loci, FDB1 and FDB2, necessary for detoxification of antimicrobial compounds produced by maize, including the γ-lactam 2-benzoxazolinone (BOA). In support of these earlier studies, microarra ...[more]

PMID: 26808652

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