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Identification of cranial-specific neural crest genes by comparative transcriptomics

ABSTRACT: We employ RNA-seq of FACS sorted cell populations to identify genes that are enriched in cranial neural crest in relationship to the trunk. Transcriptional profiling of delaminating cranial and trunk neural crest subpopulations.

ORGANISM(S): Gallus gallus

SUBMITTER: Marcos Simoes-Costa

PROVIDER: E-GEOD-75125 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Reprogramming of avian neural crest axial identity and cell fate.

Simoes-Costa Marcos M Bronner Marianne E ME

Science (New York, N.Y.) 20160601 6293

Neural crest populations along the embryonic body axis of vertebrates differ in developmental potential and fate, so that only the cranial neural crest can contribute to the craniofacial skeleton in vivo. We explored the regulatory program that imbues the cranial crest with its specialized features. Using axial-level specific enhancers to isolate and perform genome-wide profiling of the cranial versus trunk neural crest in chick embryos, we identified and characterized regulatory relationships b ...[more]

PMID: 27339986

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