Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse lymph node cells after immunizaion with pigeon cytochrome C B10.BR mice

ABSTRACT: Mice were immunized with PCC (pigeon cytochrome c). Experiment Overall Design: Lymph node cells were isolated by flow cytometry at successive timepoints after immunization for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Michael McHeyzer-Williams

PROVIDER: E-GEOD-7548 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Lymphoid reservoirs of antigen-specific memory T helper cells.

Fazilleau Nicolas N Eisenbraun Michael D MD Malherbe Laurent L Ebright Jessica N JN Pogue-Caley Rebecca R RR McHeyzer-Williams Louise J LJ McHeyzer-Williams Michael G MG

Nature immunology 20070527 7

How vaccines control the development of antigen-specific effector and memory T helper cells is central to protective immunity but remains poorly understood. Here we found that protein vaccination selected high-affinity, CXCR5+ICOS(hi) follicular B-helper T cells (T(FH) cells) that developed in draining lymphoid tissue to regulate B cell responses. In the memory phase, reservoirs of antigen-specific CXCR5+ICOS(lo) T(FH) cells persisted with less effector activity but accelerated antigen-recall ab ...[more]

PMID: 17529982

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Project description:Vaccination reduces morbidity and mortality from pneumonia but its effect on the tissue-level response to infection is still poorly understood. We evaluated pneumonia disease progression, acute phase response and lung gene expression profiles in mice inoculated intranasally with virulent gram-positive Streptococcus pneumoniae serotype (ST) 3, with and without prior immunization with pneumococcal polysaccharide ST 3 (PPS3), or co-immunization with PPS3 and with a low dose of lipopolysaccharide (LPS). Pneumonia severity was assessed in the acute phase, 5, 12, 24 and 48 h post-inoculation (p.i.) and the resolution phase of 7 days p.i. Primary PPS3 specific antibody production was upregulated and IgM binding to pneumococci increased in PPS3-immunized mice. Immunizations with PPS3 or PPS3 + LPS decreased bacterial recovery the lung and blood at 24 and 48 h and increased survival. Microarray analysis of whole lung RNA revealed significant changes in the acute phase protein serum amyloid A (SAA) between noninfected and infected mice, which were attenuated by immunization. SAA transcripts were higher in the liver and lungs of infected controls, and SAA protein was elevated in serum, but decreased in PPS3-immunized mice. Thus, during a virulent pneumonia infection, prior immunization with PPS3 in an IgM-dependent manner as well as co-immunization with PPS3 + LPS attenuated pneumonia severity and promoted resolution of infection, concomitant with significant regulation of cytokine gene expression in the lungs, and acute phase proteins in the lungs, liver and serum. Each lung RNA sample represented an individual mouse, creating biological repeats for each treatment. In-vivo treatments were as follows: non-infected lung (vehicle-immunized) control (n=5), infected lung (vehicle-immunized) control at 48 hr post-inoculation (n=5), PPS3 and LPS co-immunized lung at 48 hr post-inoculation (n=4) and PPS3 and LPS co-immunized lung at 7 days post-inoculation (n=4).

Dataset Information

Transcription profiling of mouse lymph node cells after immunizaion with pigeon cytochrome C B10.BR mice

Publications

Lymphoid reservoirs of antigen-specific memory T helper cells.

Similar Datasets

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