Unknown,Transcriptomics,Genomics,Proteomics

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BTH-responsive rice genes

ABSTRACT: Benzothiadiazole (BTH) is a so-called âplant activatorâ and protects plants from diseases by activating the salicylic-acid (SA) signaling pathway. We identified BTH-responsive genes in rice leaves 24 h after treatment using rice 44K microarray. Keywords: response to chemical treatment BTH-treated leaves were compared with mock-treated leaves using single-color method with 4 biological replicates.

ORGANISM(S): Oryza sativa

SUBMITTER: Masaki Shimono

PROVIDER: E-GEOD-7567 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast resistance.

Shimono Masaki M Sugano Shoji S Nakayama Akira A Jiang Chang-Jie CJ Ono Kazuko K Toki Seiichi S Takatsuji Hiroshi H

The Plant cell 20070629 6

Benzothiadiazole (BTH) is a so-called plant activator and protects plants from diseases by activating the salicylic acid (SA) signaling pathway. By microarray screening, we identified BTH- and SA-inducible WRKY transcription factor (TF) genes that were upregulated within 3 h after BTH treatment. Overexpression of one of them, WRKY45, in rice (Oryza sativa) markedly enhanced resistance to rice blast fungus. RNA interference-mediated knockdown of WRKY45 compromised BTH-inducible resistance to blas ...[more]

PMID: 17601827

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Project description:NPR1 is a central positive regulator of salicylic-acid (SA)-mediated defense signaling in Arabidopsis. Here, we report characterization of OsNPR1, an Oryzae sativa (rice) ortholog of NPR1, focusing on its role in blast disease resistance and identification of OsNPR1-regulated genes followed by their comparison with NPR1-regulated genes in Arabidopsis. Blast resistance tests using OsNPR1–knockdown and –overexpressing rice lines indicated that OsNPR1 plays an essential role in benzothiadiazole-induced blast resistance. Genome-wide transcript profiling using OsNPR1–knockdown lines revealed that 358 genes out of 1,228 BTH-upregulated genes and 724 genes out of 1,069 BTH-downregulated genes were OsNPR1 dependent with respect to their BTH responsiveness, indicating that OsNPR1 plays a major role in the downregulation. Inspection of OsNPR1-dependent genes revealed that many genes involved in photosynthesis and chloroplastic translation and transcription were downregulated by BTH in an OsNPR1 dependent manner, indicating that photosynthesis and chloroplast activities is coordinately suppressed by OsNPR1 in response to BTH-induced activation of SA-signaling pathway. ABA-responsive genes were also OsNPR1-dependently downregulated, suggesting antagonistic interaction of SA signaling on ABA signaling. None of 11 BTH-upregulated genes for WRKY transcription factors was OsNPR1 dependent, whereas most of those are NPR1-dependently upregulated in Arabidopsis, indicating that the role of OsNPR1 is distinct from that of NPR1 in Arabidopsis. We discuss the significance of OsNPR1-regulated gene expression in SA-regulated defense program and the role of OsNPR1 in rice SA-signaling pathway that is branched to OsNPR1- and rice WRKY45-dependent sub-pathways. mock-treated wild-type (Nipponbare) rice, benzothiadiazole (BTH)-treated wild-type rice, mock-treated WRKY45-knockdown rice (2 lines) and BTH-treated WRKY45-knockdown rice (2 lines) were analyzed in four biological replicates.

Dataset Information

BTH-responsive rice genes

Publications

Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast resistance.

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