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The major diversification of Vγ1.1+ and Vγ2+ thymocytes in mice occurs after commitment to the γδ T-cell lineage

ABSTRACT: Adult murine gammadelta thymocytes can be divided into two major subsets based on their expression of TCRVgamma1.1 or TCRVgamma2, respectively. gammadelta thymocytes mature through distinct stages characterized by CD24 and CD73 in the following order: immature uncommitted (CD24+CD73-), immature committed (CD24+CD73+) and mature (CD24-CD73+). In this dataset we have sorted gammadelta thymocytes into 6 distinct populations based on subset and developmental stage and analyzed their gene expression. These data were used to identify 3400 differentially expressed genes during gammadelta thymocyte development and to show that the two major adult gammadelta T cells follow a similar path through early development and mainly diversify after commitment to the gammadelta T cell lineage. 22 samples (6 populations 3-4 biological replicates) were analyzed for differential expression to identify genes shared between cells of the different subsets at each developmental stage.

ORGANISM(S): Mus musculus

SUBMITTER: Terkild Buus 

PROVIDER: E-GEOD-75920 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The major diversification of Vγ1.1<sup>+</sup> and Vγ2<sup>+</sup> thymocytes in mice occurs after commitment to the γδ T-cell lineage.

Buus Terkild B TB   Geisler Carsten C   Lauritsen Jens Peter H JP  

European journal of immunology 20160815 10

γδ T cells are a heterogeneous cell population with different subsets playing specialized and often opposing roles during immune responses. A key question is whether γδ thymocytes are determined for their effector function already at an early stage, before their commitment to the γδ T-cell lineage, or are instructed during their later development. Here, we show that the adult Vγ1.1<sup>+</sup> and Vγ2<sup>+</sup> γδ T-cell subsets both go through a CD73<sup>+</sup> CD24<sup>+</sup> development s  ...[more]

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