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Transcription profiling of mouse E10.5 limb bud ZPA and the rest of the E10.5 limb bud from Shhgfpcre heterozygotes separated by FACS

ABSTRACT: Comparing gene expression of cells from the E10.5 limb bud ZPA and the rest of the E10.5 limb bud from Shhgfpcre heterozygotes separated by FACS. Experiment Overall Design: 8 samples, 4 ZPA and 4 rest of the limb

ORGANISM(S): Mus musculus

SUBMITTER: Brian Harfe

PROVIDER: E-GEOD-7598 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of genes expressed in the mouse limb using a novel ZPA microarray approach.

Rock Jason R JR Lopez M Cecilia MC Baker Henry V HV Harfe Brian D BD

Gene expression patterns : GEP 20070902 1

One well-studied signaling center in the developing vertebrate limb, the zone of polarizing activity (ZPA), produces the morphogen sonic hedgehog that is necessary for normal growth and pattern formation. To identify additional factors expressed in the ZPA of the mouse limb bud, the Shhgfpcre allele was used to purify ZPA cells using fluorescence-activated cell sorting. Microarray technology was then used to identify genes whose expression was elevated in the ZPA compared to the rest of the limb ...[more]

PMID: 17911046

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