Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next generation sequencing analysis of epididymal adipose tissue (I)

ABSTRACT: We analyze the contribution of alternative splicing to the transcriptional complexity in adipose tissue and the development of diet-induced obesity. We use Next generation sequencing analysis of mice fed with a control chow diet or a high fat diet.

ORGANISM(S): Mus musculus

SUBMITTER: Yvonne Edwards

PROVIDER: E-GEOD-76133 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Next generation sequencing analysis of epididymal adipose tissue (II)

Project description:We analyze the contribution of alternative splicing to the transcriptional complexity in adipose tissue and the development of diet-induced obesity. We use Next generation sequencing analysis of eWAT from control and Nova1 and Nova2-deficient mice fed with a control diet

2016-09-06 | E-GEOD-76294 | biostudies-arrayexpress

Next generation sequencing analysis of epididymal adipose tissue (III)

2016-09-06 | E-GEOD-76317 | biostudies-arrayexpress

Next generation sequencing analysis of epididymal adipose tissue (III)

Project description:We analyze the contribution of alternative splicing to the transcriptional complexity in adipose tissue and the development of diet-induced obesity.

2016-09-06 | GSE76317 | GEO

Next generation sequencing analysis of epididymal adipose tissue (II)

Project description:We analyze the contribution of alternative splicing to the transcriptional complexity in adipose tissue and the development of diet-induced obesity.

2016-09-06 | GSE76294 | GEO

Next generation sequencing analysis of epididymal adipose tissue (I)

Project description:We analyze the contribution of alternative splicing to the transcriptional complexity in adipose tissue and the development of diet-induced obesity.

2016-09-06 | GSE76133 | GEO

RNA-seq analysis of epididymal adipose tissue from wild-type and Maf1 knockout mice

Project description:Purpose: To profile the effect of the Maf1 knockout on RNA polymerase II and RNA polymerase III transcriptomes in white adipose tissue Methods: Epididymal adipose tissue was harvested from overnight fasted 22-24 week old male mice that had been fed ad libitum on a chow diet. Total RNA was prepared from three biologically independent samples per genotype and subject to single-end directional RNA sequencing on the Illumina HiSeq platform. Sequence reads that passed quality filters were aligned against the Mm9 Mouse genome assembly using GSNAP and uniquely mapped reads were counted using HTseq-counts.

2015-05-04 | E-GEOD-65976 | biostudies-arrayexpress

Liver gene expression profile _ wild type_ CYP7A1 transgenic

Project description:Bile acids are not only physiological detergents facilitating nutrient absorption, but also signaling molecules regulating metabolic homeostasis. We reported recently that transgenic expression of CYP7A1 in mice stimulated bile acid synthesis and prevented Western diet-induced obesity, insulin resistance and hepatic steatosis. The aim of this experiment is to determine the impact of induction of hepatic bile acid synthesis on liver metabolism by determining hepatic gene expression profile in CYP7A1 transgenic mice. CYP7A1 transgenic mice and wild type control mice were fed either standard chow diet or high fat high cholesterol Western diet for 4 month. Hepatic gene expressions were measured by microarray analysis. Our results indicate that hepatic bile acid synthesis is closely linked to cholesterogenesis and lipogenesis, and maintaining bile acid homeostasis is improtant in hepatic metabolic homeostasis. Male aged matched (~ 12-14 weeks) CYP7A1 transgenic mice and their wild type control littermates were fed a standard chow diet or a high fat (42%) high cholesterol (0.2%) diet (Harlan Teklad #88137) for 4 month Four groups (4 mice/group) are included in the experiments: Group 1: WT _ Chow Group 2: CYP7A1-tg + chow Group 3: WT + Western diet Group 4: CYP7A1-tg _ Western diet Total liver mRNA was isolated with a RNeasy kit (Qiagen) and used for microarray analysis.

2013-08-15 | E-GEOD-38872 | biostudies-arrayexpress

Temporal gene expression profiling of murine epididymal adipose with different diet regimens [epididymal adipose]

Project description:In the present study, we used C57BL/6J mouse, as a model for studying diet-induced diabetes and obesity, since this strain accumulates adipose tissue mass, insulin resistance, hyper-insulinemia, and hyper-lipidemia similar to humans fed on an high-fat high-sugar diet (HFHSD).

2014-11-12 | GSE63170 | GEO

SYSTEMATIC TRANSCRIPTIONAL PROFILING OF THE LIVER, HEART, SKELETAL MUSCLE, AND ADIPOSE TISSUE IN INSULIN RESISTANT, DIET-INDUCED OBESE C57BL/6J MOUSE

Project description:Following 20wk of either chow or high fat feeding, tissues were collected from C57BL/6J mice. Gene expression in four tissues that have been associated with obesity-related metabolic complications: white adipose tissue (WAT), skeletal muscle, the liver, and the heart were examined. Data suggest that some tissues may be attempting to reduce the metabolic perturbations that occur in the early stages of obesity. Also, many identified genes were associated with a variety of disorders, thereby serving as potential links between obesity and related health risks. After 3 wk of weaning, male C57BL/6J littermates were randomly separated into control (n=6) and treatment (n=6) groups for each tissue. After an acclimatization period of 1wk, animals were given free access to a chow diet (23% protein, 21% fat, and 55% carbohydrate) or a high fat (HF) diet (15% protein, 59% fat, and 26% carbohydrate) for 20wk. Following the feeding period, animals were fasted for 6h, anesthetized with pentobarbital, and weighed. Blood glucose was assessed in anesthetized mice. Non-esterified fatty acids and immunoreactive insulin was assayed with a double antibody method. Samples of WAT, the liver, gastrocnemius muscle and heart were obtained, frozen with liquid nitrogen, and stored at -80oC until subsequent use. Tissue samples were homogenized at short intervals of time and total RNA was isolated using cold TRIzol (Invitrogen, Ontario, Canada) to prevent RNA degradation. Cdna was prepared to perform the microarrays. For every treatment sample and its corresponding control, two microarrays were performed in a dye-swap normalization experiment. Mouse M7K microarray chip from the Southern Alberta Microarray Facility (University of Calgary, Calgary, AB) were used (GEO Accession: Platform GPL3965).

2011-03-01 | E-GEOD-26029 | biostudies-arrayexpress

Analysis of murine epididymal adipose tissue

Project description:Functional genomic analysis of epididymal adipose tissue extracted from polygenic obese mice fed either a control (1% linoleic acid) or treatment (1% trans 10, cis 12 conjugated linoleic acid) diet after five or fourteen days. Keywords = Obesity Keywords = conjugated linoleic acid Keywords = polygenic obese mice Keywords = adiposity Keywords = lipid metabolism Keywords = apoptosis Keywords: repeat sample

2004-07-23 | GSE1580 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data