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Reduced representation bisulfite sequencing data of mouse genome

ABSTRACT: RRBS was used to analyse global imprinting gene methylation pattern of offspring from the in vitro derived spermatids like cells and normal control Global imprinting gene methylation pattern comparision of two offspring (one male and one female) from the in vitro derived spermatids like cells and two normal control mice (one male and one female)

ORGANISM(S): Mus musculus

SUBMITTER: Quan Zhou

PROVIDER: E-GEOD-76238 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Complete Meiosis from Embryonic Stem Cell-Derived Germ Cells In Vitro.

Zhou Quan Q Wang Mei M Yuan Yan Y Wang Xuepeng X Fu Rui R Wan Haifeng H Xie Mingming M Liu Mingxi M Guo Xuejiang X Zheng Ying Y Feng Guihai G Shi Qinghua Q Zhao Xiao-Yang XY Sha Jiahao J Zhou Qi Q

Cell stem cell 20160225 3

In vitro generation of functional gametes is a promising approach for treating infertility, although faithful replication of meiosis has proven to be a substantial obstacle to deriving haploid gamete cells in culture. Here we report complete in vitro meiosis from embryonic stem cell (ESC)-derived primordial germ cells (PGCLCs). Co-culture of PGCLCs with neonatal testicular somatic cells and sequential exposure to morphogens and sex hormones reproduced key hallmarks of meiosis, including erasure ...[more]

PMID: 26923202

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