Circulating microRNA screening of high-risk individuals enrolled in COSMOS lung cancer screening trial.
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ABSTRACT: Lung cancer is the leading cause of cancer death worldwide. Low-dose computed tomography screening (LDCT) was recently shown to anticipate the time of diagnosis, thus reducing lung cancer mortality. We identifed a serum microRNA signature (the miR-Test) that could identify the optimal target population for LDCT screening. Here, we performed a large-scale validation study of the miR-Test in high-risk individuals enrolled in the Continuous Observation of Smoking Subjects (COSMOS) lung cancer screening program. RT-qPCR of circulating microRNA purified from serum samples. Trizol-LS and miRNEASY Mini kit (Qiagen) were used for miRNA purification. Custom TaqMan® Low Density Array microRNA Custom Panel (Life Technologies) was used to screen serum circulating microRNA.
ORGANISM(S): Homo sapiens
SUBMITTER: Fabrizio Bianchi
PROVIDER: E-GEOD-76462 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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