Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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BCL6 ChIP on chip using a Nimblegen custom tiling array


ABSTRACT: To determine whether BCL6 binds to the certain locus we performed genomic localization studies by chromatin immunoprecipitations (ChIP) using a densely tiled custom oligonucleotide microarray covering the genomic loci of different genes. Keywords: ChIP-chip, Transcription Factor localization The experiment was performed in Ramos cell. Triplicate ChIP with BCL6 or actin (negative control) antibodies were cohybridized to the arrays versus the respective input chromatin.The fold enrichment for each oligonucleotide was calculated as the ratio of Cy5 vs. Cy3. Any peaks involving >5 oligonucleotides and with > 2.5 fold enrichment were considered potentially positive hits.

ORGANISM(S): Homo sapiens

SUBMITTER: Jose Polo 

PROVIDER: E-GEOD-7673 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Bcl-6 mediates the germinal center B cell phenotype and lymphomagenesis through transcriptional repression of the DNA-damage sensor ATR.

Ranuncolo Stella Maris SM   Polo Jose M JM   Dierov Jamil J   Singer Michael M   Kuo Tracy T   Greally John J   Green Roland R   Carroll Martin M   Melnick Ari A  

Nature immunology 20070610 7


Antibody specificity and diversity is generated in B cells during germinal center maturation through clonal expansion while they undergo class-switch recombination and somatic hypermutation. Here we demonstrate that the transcriptional repressor Bcl-6 mediates this phenotype by directly repressing ATR in centroblasts and lymphoma cells. ATR is critical in replication and DNA damage-sensing checkpoints. Bcl-6 allowed B cells to evade ATR-mediated checkpoints and attenuated the response of the B c  ...[more]

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