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Pho and Ph, H3K27me3 and Engrailed ChIP-seq data in Drosophila third instar larval brains and disks

ABSTRACT: Genome-wide binding profile of PcG proteins Pho and Ph, H3K27me3 and Engrailed in Drosophila third instar larval brains and disks, in duplicate, using Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed. Drosophila third instar larval brains and disks were dissected and fixed with 2% HCHO before sonication. After sonication, chromatin immunoprecipitation using anti-Pho, -Ph, -H3K27me3, and -Engrailed antibodies was carried out.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: sandip de

PROVIDER: E-GEOD-76892 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Combgap contributes to recruitment of Polycomb group proteins in Drosophila.

Ray Payal P De Sandip S Mitra Apratim A Bezstarosti Karel K Demmers Jeroen A A JA Pfeifer Karl K Kassis Judith A JA

Proceedings of the National Academy of Sciences of the United States of America 20160321 14

Polycomb group (PcG) proteins are responsible for maintaining the silenced transcriptional state of many developmentally regulated genes. PcG proteins are organized into multiprotein complexes that are recruited to DNA via cis-acting elements known as "Polycomb response elements" (PREs). In Drosophila, PREs consist of binding sites for many different DNA-binding proteins, some known and others unknown. Identification of these DNA-binding proteins is crucial to understanding the mechanism of PcG ...[more]

PMID: 27001825

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