Unknown,Transcriptomics,Genomics,Proteomics

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Control of progenitor cell positioning and organ patterning during embryogenesis

ABSTRACT: The precise positioning of organ progenitor cells constitutes an essential, yet poorly understood step in organ development and function. Using primordial germ cells and the process of gonadogenesis as anÂ in vivoÂ model, we present the developmental mechanisms facilitating the maintenance of a motile progenitor cell population at the site where the organ develops. We find that the action of repulsive cues coupled with the generation of physical barriers confine the cells to the correct bilateral positions within the embryo. Using computer particle-based simulations we could also demonstrate the role of reflecting barriers, from which cells turn away upon contact, and the importance of proper level of cell-cell interaction for maintaining the tight cell clusters and their correct positioning at the target region. The cellular mechanisms operating during those events were determined using high-resolution live imaging microscopy. This analysis revealed cell polarity change upon interaction with the physical barrier and the establishment of compact clusters that involves increased cell-cell interaction as a result of collapse of cell protrusions and enhanced adhesion. The combination of these developmental and cellular RNA profiling in zebrafish embryos of 7 hpf and 36 hpf in sorted germ cells and randomly sorted somatic cells

ORGANISM(S): Danio rerio

SUBMITTER: Katsiaryna Tarbashevich

PROVIDER: E-GEOD-77077 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Gene set and sub-network enrichment analysis provides an integrated approach for identifying gene regulatory networks underlying development. Anuran development is a coordinated process, and levels of mRNA are first dominated by maternally deposited genes, followed by active transcription of embryonic genes as the embryo undergoes morphogenesis and organ formation. The objectives of this study were to characterize early gene regulatory networks underlying Silurana tropicalis development. A custom Agilent 4 x 44 K microarray was developed to characterize networks during early development (1, 17, 36, 96 hpf). Cluster analysis revealed that each stage showed unique gene expression profiles and that 1 hpf was most different than the other three stages. There were > 8000 unique gene probes (p<0.01, FDR = 5%) that were differentially expressed between 1 hpf (2 cell stage) and 17 hpf and > 2000 gene probes differentially expressed between 36 hpf and 96 hpf. Genes higher in abundance (>100-fold) at 1 hpf compared to 17 hpf included oocyte-specific histone RNA stem-loop-binding protein 2, mitogen-activated protein kinase 14, and cyclin B5, suggesting these transcripts are maternally inherited or actively transcribed at fertilization. Gene ontology revealed that genes involved in nucleosome assembly, cell division, pattern specification, neurotransmission, and general metabolism were increasingly regulated throughout development, consistent with active development. In the period between 17-36 hpf, gene networks that play a role in organogenesis and organ function, including those related to the heart (heart morphogenesis, central nervous system (olfactory bulb development, dopamine metabolism), and kidney (renal reabsorption, water balance) were activated while between 36-96 hpf, networks involving in gut development, immune responses, lipid metabolism, hormone signaling, and brain development were prevalent. This study increases understanding of the spatiotemporal S. tropicalis embryonic development using gene regulatory networks.

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Control of progenitor cell positioning and organ patterning during embryogenesis

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