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Single-cell RNA-seq of Ecad positive PyMT bulk tumor cells

ABSTRACT: In this study we studied the presence of tumor cells that underwent epithelial-to-mesenchymal transition within polyoma middle T antigen (PyMT) breast tumors. For this we dissociated tumors and isolated Ecad positive tumor cells by FACS sorting. We confirmed that PyMT tumors contain a small set of tumor cells that have undergone EMT in the primary tumor and that E-cadherin can be used as a marker on single cell level for mesenchymal status in this model. We isolated primary tumors from mice, dissociated the tumors and FACS-sorted for single Ecad positive tumor cells, after this we performed single cell sequencing of the cells.

ORGANISM(S): Mus musculus

SUBMITTER: Jean-Charles Boisset

PROVIDER: E-GEOD-77107 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Plasticity between Epithelial and Mesenchymal States Unlinks EMT from Metastasis-Enhancing Stem Cell Capacity.

Beerling Evelyne E Seinstra Daniëlle D de Wit Elzo E Kester Lennart L van der Velden Daphne D Maynard Carrie C Schäfer Ronny R van Diest Paul P Voest Emile E van Oudenaarden Alexander A Vrisekoop Nienke N van Rheenen Jacco J

Cell reports 20160303 10

Forced overexpression and/or downregulation of proteins regulating epithelial-to-mesenchymal transition (EMT) has been reported to alter metastasis by changing migration and stem cell capacity of tumor cells. However, these manipulations artificially keep cells in fixed states, while in vivo cells may adapt transient and reversible states. Here, we have tested the existence and role of epithelial-mesenchymal plasticity in metastasis of mammary tumors without artificially modifying EMT regulators ...[more]

PMID: 26947068

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