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RecG Directs DNA Synthesis During Double-Strand Break Repair [Chromosomal marker frequency analysis]

ABSTRACT: In the bacterium Escherichia coli, RecG directs DNA synthesis during the repair of DNA double-strand breaks by homologous recombination. Chromosomal marker frequency analysis (MFA) following induction of a DSB in the absence and presence of RecG

ORGANISM(S): Escherichia coli

SUBMITTER: Benura Azeroglu

PROVIDER: E-GEOD-77183 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RecG Directs DNA Synthesis during Double-Strand Break Repair.

Azeroglu Benura B Mawer Julia S P JS Cockram Charlotte A CA White Martin A MA Hasan A M Mahedi AM Filatenkova Milana M Leach David R F DR

PLoS genetics 20160212 2

Homologous recombination provides a mechanism of DNA double-strand break repair (DSBR) that requires an intact, homologous template for DNA synthesis. When DNA synthesis associated with DSBR is convergent, the broken DNA strands are replaced and repair is accurate. However, if divergent DNA synthesis is established, over-replication of flanking DNA may occur with deleterious consequences. The RecG protein of Escherichia coli is a helicase and translocase that can re-model 3-way and 4-way DNA str ...[more]

PMID: 26872352

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