Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: David Wong
PROVIDER: E-GEOD-7760 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Hu Zhanzhi Z Zimmermann Bernhard G BG Zhou Hui H Wang Jianghua J Henson Bradley S BS Yu Weixia W Elashoff David D Krupp Guido G Wong David T DT
Clinical chemistry 20080320 5
<h4>Background</h4>The application of global gene expression profiling to saliva samples is hampered by the presence of partially fragmented and degraded RNAs that are difficult to amplify and detect with the prevailing technologies. Moreover, the often limited volume of saliva samples is a challenge to quantitative PCR (qPCR) validation of multiple candidates. The aim of this study was to provide proof-of-concept data on the combination of a universal mRNA-amplification method with exon arrays ...[more]