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Nucleosome Positioning Dynamics Through the Yeast Metabolic Cycle

ABSTRACT: We present Micrococcal Nuclease digestion maps of S. cerevisiae through the progression of the Yeast Metabolic Cycle. We demonstrate that nucleosome positions at many promoters are dynamic, and remodeling events at promoters have significant consequences with respect to gene expression. Examination of nucleosome positions and transcriptional output through metabolic oscillations in budding yeast.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Iestyn Whitehouse

PROVIDER: E-GEOD-77631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nucleosome repositioning underlies dynamic gene expression.

Nocetti Nicolas N Whitehouse Iestyn I

Genes & development 20160310 6

Nucleosome repositioning at gene promoters is a fundamental aspect of the regulation of gene expression. However, the extent to which nucleosome repositioning is used within eukaryotic genomes is poorly understood. Here we report a comprehensive analysis of nucleosome positions as budding yeast transit through an ultradian cycle in which expression of >50% of all genes is highly synchronized. We present evidence of extensive nucleosome repositioning at thousands of gene promoters as genes are ac ...[more]

PMID: 26966245

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